Rabbit anti-Human CD42b Monoclonal Antibody for Flow Cytometry - P07359

Core Product Specifications and Parameters

Biological Background: CD42b / GP1BA Function and Localization

• CD42b, also known as platelet glycoprotein Ib alpha chain (GP1BA), is a surface membrane protein of platelets.
• It plays a crucial role in primary hemostasis by mediating platelet adhesion to von Willebrand factor (vWF) at sites of vascular injury, facilitating platelet plug formation.
• Subcellularly, it is an integral membrane protein with a transmembrane domain, expressed on the platelet surface.
• The protein undergoes post-translational modifications including sulfation, phosphorylation, and glycosylation.
• Mutations in GP1BA are linked to Bernard-Soulier syndrome and von Willebrand disease, as indicated by disease variant associations.
• The canonical isoform has a calculated molecular weight of approximately 71.5 kDa, though the product specification lists 72 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 15-531 of human CD42b, corresponding to the extracellular domain. This design may favor antibodies recognizing native, cell-surface conformations suitable for flow cytometry.
• For flow cytometry (FC), consider titrating the antibody to determine optimal staining indices. Validate the antibody's performance with appropriate positive (e.g., platelets, platelet-rich plasma) and negative (e.g., platelet-depleted samples) controls.
• Cross-reactivity is reported for human CD42b. If working with other species, verify sequence homology and cross-reactivity experimentally.
• As the antibody is conjugated to , ensure compatibility with your instrument's laser/filter settings (excitation max ~488 nm, emission ~515 nm). Protect from light to prevent photobleaching.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with comprehensive raw material solutions. For CD42b-related research in hemostasis and platelet biology, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Streamline your development from concept to clinic with CamelBio's integrated procurement services.