Rabbit anti-Human CD40 Monoclonal Antibody for Flow Cytometry - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40 (Tumor necrosis factor receptor superfamily member 5), also known as Bp50, CDw40, and TNFRSF5, is a member of the TNF receptor superfamily and functions as the receptor for CD40 ligand (CD40LG/TNFSF5).
• Upon ligand binding, CD40 transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:31331973). Related references: PMID:31331973
• CD40 is primarily expressed on B cells and in primary carcinomas, as reported in tissue specificity studies.
• The protein exists as a cell surface receptor but can also be secreted, as indicated by subcellular localization annotations.
• CD40 undergoes alternative splicing, producing multiple isoforms; the full-length protein contains a signal peptide, an extracellular domain with cysteine-rich repeats, a transmembrane helix, and a cytoplasmic tail.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, which are critical for receptor stability and function.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 21-193), suggesting this antibody recognizes the CD40 ectodomain; confirm epitope specificity in your assay.
• For flow cytometry, include appropriate isotype controls and dead cell exclusion dyes to ensure specific staining.
• Optimize antibody concentration by titration to achieve the best staining index; typical concentrations range from 0.1 to 1 µg per test.
• If considering other applications, validate the antibody in your model system as it is recommended for FC only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials and technical services, covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies like this anti-CD40 clone, bulk ancillary reagents, and rare target raw-material sourcing. Whether advancing immunology research or developing CD40-targeted diagnostics, CamelBio streamlines your supply chain with quality-assured materials and expert support.