Rabbit anti-Human CD40 Monoclonal Antibody for FC - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40 is a member of the tumor necrosis factor receptor superfamily (TNFRSF5), also known as B-cell surface antigen CD40, Bp50, CDw40, and CD40L receptor.
• Functions as a receptor for TNFSF5/CD40LG, transducing TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. Related references: PMID:31331973
• Localizes predominantly to the cell membrane; a secreted isoform has also been reported.
• Expressed primarily on B-cells and in primary carcinomas.
• Contains multiple structural features including disulfide bonds, a signal peptide, a transmembrane helix, and glycosylation sites, which are critical for receptor stability and ligand interaction.
• Alternative splicing generates distinct isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21-193) of human CD40, facilitating the generation of antibodies that recognize the cell surface receptor, suitable for flow cytometry.
• For FC applications, optimal antibody concentration should be determined by titration; include appropriate isotype controls and viability dyes.
• The conjugate eliminates the need for secondary antibodies, reducing background and simplifying the staining protocol.
• Validate antibody performance in the relevant biological sample system, such as human peripheral blood mononuclear cells (PBMCs) or B-cell lines, under experimental conditions.

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CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal antibodies like this anti-CD40 clone, bulk ancillary reagents, and rare target sourcing to accelerate your diagnostic development. Our supply capabilities support immunological and oncological research areas, ensuring seamless integration from target selection to clinical application. Let CamelBio bridge your raw-material needs, from concept to clinic.