Rabbit anti-Human CD40 Monoclonal Antibody for FC - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40 is the receptor for TNFSF5/CD40LG (PubMed:31331973). Related references: PMID:31331973
• Upon ligand binding, it transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to immunoglobulin secretion.
• CD40 is predominantly expressed on B-cells and in primary carcinomas.
• It localizes to the cell membrane and can also be secreted.
• The protein undergoes glycosylation and disulfide bond formation, characteristic of cell surface receptors.
• As a member of the tumor necrosis factor receptor superfamily, CD40 plays a critical role in immune cell activation and survival.
• Alternative splicing of CD40 generates multiple isoforms with potentially distinct functions.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 21-193), ensuring recognition of the native receptor on the cell surface.
• For flow cytometry (FC), titrate the antibody to determine the optimal working concentration for your cell population of interest.
• Validate the antibody in human B-cell lines or primary B-cells, where CD40 is endogenously expressed.
• As CD40 can be cleaved and secreted, consider using a membrane-specific staining protocol with appropriate viability dyes.
• When multiplexing with other fluorophores, ensure minimal spectral overlap with (excitation/emission similar to Alexa Fluor 647, Cy5).
• If using for other applications, note that the conjugate may have limitations in non-FC assays; test performance after conjugation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Rabbit anti-Human CD40 mAb targets a critical immune checkpoint, and CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your CD40-related IVD development and research.