Rabbit anti-Human CD40 mAb - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• Tumor necrosis factor receptor superfamily member 5, also known as CD40, Bp50, CDW40, and TNFRSF5, is encoded by the CD40 gene in humans.
• Acts as receptor for TNFSF5/CD40LG, transducing TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. Related references: PMID:31331973
• Localizes primarily to the cell membrane and is also secreted, with expression found in B-cells and in primary carcinomas.
• Contains a signal peptide, a transmembrane helix, and multiple repeats; undergoes post-translational modifications including glycosylation and disulfide bond formation.
• Alternative splicing generates multiple transcript variants, and mutations in CD40 are associated with disease variants.
• Classified under gene ontology terms such as immunity, receptor, membrane, and secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of human CD40 (amino acids 21-193), suggesting the antibody targets an epitope in the ligand-binding region.
• For flow cytometry (FC) applications, titrate the antibody to determine the optimal concentration for your specific experimental setup and cell type (e.g., human B cells or carcinoma cell lines).
• Verify staining specificity using appropriate positive (e.g., CD40-expressing B cells) and negative controls, as CD40 expression can vary across cell lines and activation states.
• Note that the predicted molecular weight of CD40 is approximately 30.6 kDa (UniProt: P25942), but glycosylation may cause the observed band to shift to ~31-44 kDa in western blotting; however, this antibody is validated for FC only.
• Since this antibody is conjugated to , avoid freeze-thaw cycles and store protected from light to maintain fluorescence stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials and technical services, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your diagnostic development. From concept to clinic, we provide comprehensive solutions for CD40-targeted research and immunoassay development. Our extensive supply network ensures reliable access to high-quality raw materials for immunology and oncology applications.