Rabbit anti-Human CD39/ENTPD1 mAb for FC - P49961

Core Product Specifications and Parameters

Biological Background: Ectonucleoside triphosphate diphosphohydrolase 1 (CD39/ENTPD1) Function and Localization

• Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1), also known as CD39, ATPDase, NTPDase-1, and lymphoid cell activation antigen, is a member of the ectonucleotidase family.

• Catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) to nucleoside monophosphates (NMPs) in two successive phosphate-removal steps (Probable). Related references: PMID:8529670, PMID:8626624, PMID:8955160

• Plays a key role in regulating extracellular nucleotide levels; by hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, CD39 blocks platelet aggregation and supports blood flow. Related references: PMID:8955160, PMID:8996251

• Localizes to the plasma membrane and associates with caveolae, consistent with its ectonucleotidase function.

• Predominantly expressed on activated lymphoid cells, with additional expression in endothelial tissues, placenta, lung, skeletal muscle, and kidney. Related references: PMID:7930580, PMID:8955160

• Post-translational modifications include glycosylation, disulfide bond formation, and palmitoylation, which may influence protein stability and localization.

• Mutations in ENTPD1 are associated with hereditary spastic paraplegia (SPG64), linking CD39 dysfunction to neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 255–442 of human CD39, which lies within the extracellular domain containing the catalytic site. Consider this epitope location when designing blocking or epitope mapping experiments.
• For flow cytometry, verify cell surface expression of CD39 on your target population and include appropriate isotype controls to ensure specificity. Use live-cell staining unless intracellular detection is required; CD39 is primarily surface-exposed.
• If combining with other fluorophores, confirm minimal spectral overlap and compensate appropriately. Perform titration experiments to determine the optimal antibody concentration.
• Validate the antibody in your specific cell system, as CD39 expression can vary with activation state and tissue origin.

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CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for immune-related targets like CD39. We also offer rare target raw-material sourcing to accelerate your immunoassay and diagnostic development pipelines.