Rabbit anti-Human CD39/ENTPD1 mAb for FC - P49961

Core Product Specifications and Parameters

Biological Background: CD39/ENTPD1 Function and Localization

• CD39/ENTPD1 is an ectonucleotidase that sequentially hydrolyzes nucleoside triphosphates (NTPs) to nucleoside diphosphates (NDPs) and then to nucleoside monophosphates (NMPs), regulating extracellular nucleotide levels. Related references: PMID:8529670, PMID:8626624, PMID:8955160
• By degrading proinflammatory ATP and platelet-activating ADP to AMP, CD39 inhibits platelet aggregation and promotes blood flow, highlighting its role in vascular homeostasis. Related references: PMID:8955160, PMID:8996251
• The ENTPD1 gene encodes CD39, a membrane glycoprotein that acts as an ecto-ATP diphosphohydrolase, also known as NTPDase1 and lymphoid cell activation antigen.
• CD39 is localized to the cell membrane, especially in caveolae, positioning it to efficiently modulate extracellular nucleotide signaling.
• It is highly expressed in activated lymphoid cells, endothelial tissues, placenta, lung, skeletal muscle, and kidney, consistent with immunological and vascular functions.
• The protein undergoes alternative splicing, glycosylation, and palmitoylation, and contains key disulfide bonds essential for its structure.
• Mutations in ENTPD1 are associated with autosomal recessive hereditary spastic paraplegia (SPG64) and neurodegeneration, underscoring its importance in neuronal health.

Experimental Guidance and Technical Tips

• This -conjugated antibody is designed for direct flow cytometric detection, eliminating the need for secondary antibodies. Consider titrating the antibody for optimal signal-to-noise ratio in human samples.
• The immunogen corresponds to amino acids 255–442 of human CD39, an extracellular domain accessible on live cells; thus, this antibody is suitable for surface staining without permeabilization.
• For best results, use fresh human peripheral blood mononuclear cells or cultured cell lines expressing CD39; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with premium IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this CD39/ENTPD1 product. We support ectonucleotidase and immune-oncology research with bulk ancillary reagents and custom sourcing solutions for rare targets. Rely on CamelBio for consistent quality and seamless procurement from discovery to clinical application.