Rabbit anti-Human CD38 mAb - P28907

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 is a multifunctional transmembrane glycoprotein (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1) that synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion. Related references: PMID:7961800 and PMID:8253715
• CD38 functions as a receptor binding CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and migration across endothelial barriers. Related references: PMID:9551996
• Involved in regulating crucial dendritic cell functions at the mature stage, including CCL21-driven migration, survival, and Th1-polarizing activity. Related references: PMID:16293598
• In lamina propria T lymphocytes, CD38/CD31 cognate interactions initiate a signaling pathway resulting in activation of LCK and LAT, followed by cytokine release. Related references: PMID:11259373
• Preferentially transforms 2'-phospho-cADPR into NAADP(+) at both pH 5.0 and 7.4, and also possesses cADPR hydrolase activity. Related references: PMID:16690024
• Subcellular localization: prominently expressed on the cell surface and cell membrane.
• Tissue specificity: highly expressed in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma, and neuroblastoma.
• Post-translational modifications include disulfide bond formation and glycosylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 43-300) encompasses the extracellular domain, making this antibody suitable for detecting surface CD38 in live-cell flow cytometry without permeabilization.
• Perform titration and use appropriate isotype controls to optimize flow cytometry staining conditions.
• CD38 expression levels can vary between cell types and disease states; validate the antibody in your specific experimental system.
• Ensure compatibility of the conjugate with your cytometer's laser and filter configuration, and perform compensation as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials and technical services to diagnostic manufacturers and research laboratories. For CD38 research in immunology, oncology, and diabetes, we offer validated antibody pairs, recombinant proteins, and bulk ancillary reagents. Our sourcing experts can also assist with custom antibody production and rare-target reagent procurement.