Rabbit anti-Human CD370/CLEC9A Monoclonal Antibody for Flow Cytometry - Q6UXN8

Core Product Specifications and Parameters

Biological Background: CD370/CLEC9A Function and Localization

• Protein identity and aliases: CD370, also known as C-type lectin domain family 9 member A (CLEC9A), DNGR-1, and UNQ9341, is encoded by the CLEC9A gene in humans.
• Core function as an endocytic receptor: CLEC9A functions as an endocytic receptor on a small subset of myeloid cells, specialized for the uptake and processing of material from dead cells. It mediates the cross-presentation of dead cell-associated antigens in a Syk-dependent manner.
• Ligand recognition mechanism: The receptor recognizes the filamentous form of actin (F-actin) in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, which become exposed upon cell membrane damage.
• Tissue and cellular expression: Its expression is highly restricted in peripheral blood to the surface of BDCA3+ dendritic cells and on a small subset of CD14+ and CD16− monocytes.
• Subcellular localization: CLEC9A is a membrane protein, consistent with its role as a cell surface receptor. It contains a single-pass transmembrane helix and a signal-anchor sequence, classifying it as a type II membrane protein.
• Structural and functional features: The protein belongs to the C-type lectin family and contains disulfide bonds and N-linked glycosylation sites, characteristic of membrane receptors involved in endocytosis. Its molecular weight is approximately 27 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 110–241 of the extracellular domain (NP_997228.1), which contains the carbohydrate-recognition domain. This region is likely to be accessible on live cells, supporting its use in flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type. Include an appropriate isotype control and consider using Fc receptor blocking reagent to reduce nonspecific binding.
• Since CLEC9A is an endocytic receptor, binding of the antibody may trigger internalization. To minimize potential internalization, perform staining steps at 4°C in the presence of sodium azide if feasible.
• Validate the antibody on a known positive sample, such as BDCA3+ dendritic cells or CD14+ monocytes, to confirm specificity. The restricted expression pattern makes it a useful marker for immunophenotyping studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, supporting every stage from concept to clinic. This anti-CLEC9A monoclonal antibody, targeting a key dendritic cell receptor, fits seamlessly into immunophenotyping and immune monitoring workflows. Explore our extensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, as well as custom development services to meet your diagnostic and research needs.