Rabbit anti-Human CD370/CLEC9A Monoclonal Antibody for Flow Cytometry - Q6UXN8

Core Product Specifications and Parameters

Biological Background: CD370/CLEC9A Function and Localization

• CD370/CLEC9A (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane receptor containing a single C-type lectin-like domain.
• It functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells.
• Recognizes filamentous actin associated with particular actin-binding domains of cytoskeletal proteins, including spectrin, which become exposed upon cell membrane damage.
• Mediates the cross-presentation of dead-cell associated antigens in a Syk-dependent manner, linking innate sensing of cell death to adaptive immunity.
• Subcellular localization: predominantly expressed on the cell membrane, anchored via a signal-anchor and a single transmembrane helix.
• Tissue specificity: highly restricted expression on BDCA3+ dendritic cells and a small subset of CD14+ CD16- monocytes in peripheral blood.
• Post-translational modifications: predicted glycoprotein with disulfide bonds, consistent with its extracellular C-type lectin domain.
• The predicted molecular weight of the unmodified protein is approximately 27.3 kDa, with the product detecting a band at 27 kDa (presumably the glycosylated form may migrate slightly differently).

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , allowing for immediate flow cytometry without secondary staining steps.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Validate performance in human peripheral blood mononuclear cells (PBMCs) or enriched dendritic cell populations.
• Include appropriate isotype control and viability dye to ensure accurate gating on live, CD370+ cells.
• For fixation, test compatibility as fluorescence may be affected by common fixatives; consider testing under both live and fixed conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including validated monoclonal antibodies like this anti-CD370/CLEC9A clone. Our comprehensive portfolio supports dendritic cell biology, immune monitoring, and dead cell antigen cross-presentation research. We offer one-stop access to antibody pairs, bulk ancillary reagents, and rare target sourcing, streamlining your journey from concept to clinic.