Rabbit anti-Human CD370/CLEC9A mAb - Q6UXN8

Core Product Specifications and Parameters

Biological Background: CD370/CLEC9A Function and Localization

• CLEC9A, also known as CD370 or DNGR-1, is a C-type lectin domain family member that acts as an endocytic receptor on a specialized subset of myeloid cells.
• It recognizes filamentous actin in complexes with actin-binding domains of cytoskeletal proteins such as spectrin, which become exposed upon cell membrane damage.
• This recognition enables the receptor to mediate the uptake and cross-presentation of dead-cell-associated antigens in a Syk kinase-dependent manner.
• In peripheral blood, CLEC9A expression is highly restricted to BDCA3+ dendritic cells and a small subset of CD14+ CD16- monocytes.
• The protein is a single-pass type II membrane glycoprotein containing a C-type lectin domain, and is annotated with keywords including receptor, endocytosis, and transmembrane helix.
• Its molecular weight is approximately 27 kDa, consistent with its predicted size from the primary sequence.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a far-red fluorophore, and is validated for flow cytometry (FC). Ensure proper compensation and panel design.
• The immunogen covers amino acids 110-241 of the extracellular domain, which should permit detection of cell surface CLEC9A on live cells.
• When staining rare dendritic cell populations, include optimal blocking steps and consider using an isotype control to assess background.
• Optimal antibody concentration and staining conditions must be determined empirically for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw material sourcing, offering a broad portfolio of validated antibodies, proteins, and ancillary reagents. For researchers investigating dendritic cell biology and immune monitoring, we provide reliable tools like anti-CLEC9A antibodies to support your assay development and clinical translation efforts. Contact us to explore customized sourcing solutions that accelerate your path from concept to clinic.