Rabbit anti-Human CD369/Dectin-1 Monoclonal Antibody for Flow Cytometry - Q9BXN2

Core Product Specifications and Parameters

Biological Background: CD369/Dectin-1 (CLEC7A) Function and Localization

• CLEC7A encodes the C-type lectin receptor Dectin-1, a key pattern recognition receptor (PRR) that specifically recognizes β-1,3- and β-1,6-linked glucans on pathogenic bacteria and fungi. Related references: PMID:11567029 PMID:12423684
• Upon β-glucan engagement, Dectin-1 signals through its cytoplasmic ITAM motif to recruit SYK, initiating downstream CARD9-BCL10-MALT1 (CBM) complex assembly that leads to NF-κB and p38 MAPK activation.
• This signaling cascade promotes the production of pro-inflammatory cytokines and chemokines, enhances reactive oxygen species generation, and is crucial for antifungal innate immunity.
• Dectin-1 directly mediates the phagocytosis of fungal pathogens such as Candida albicans conidia. Related references: PMID:17230442
• Beyond myeloid cells, Dectin-1 also binds to T cells and can stimulate T-cell proliferation, indicating a role in adaptive immunity.
• The protein resides primarily at the cell membrane but can also be detected in the cytoplasm; it is highly expressed in peripheral blood leukocytes, dendritic cells, and tissues like spleen, bone marrow, and lung.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation; alternative splicing yields isoforms with distinct molecular weights (observed at 5, 8, and 13–27 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 66–247 of human Dectin-1, which encompasses the extracellular C-type lectin domain, making this -conjugated antibody well suited for detecting surface Dectin-1 by flow cytometry.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type and using appropriate isotype controls to ensure specific staining.
• Validate staining in human peripheral blood mononuclear cells or dendritic cell preparations, where Dectin-1 is highly expressed, before applying to experimental samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing access to rigorously developed monoclonal antibodies like this anti-CD369/Dectin-1 clone for researchers and diagnostic manufacturers. In addition to flow-cytometry-ready antibody conjugates, we offer validated antibody pairs, optimized bulk reagents, and assistance in sourcing rare-target raw materials. Our one-stop model streamlines procurement from lead identification to commercial scale—ensuring you have a reliable supply chain for your innate immunity and infectious disease assay development needs.