Rabbit anti-Human CD369/Dectin-1 mAb for FC - Q9BXN2

Core Product Specifications and Parameters

Biological Background: CD369/Dectin-1 Function and Localization

• Serves as a pattern recognition receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans from pathogenic bacteria and fungi. Related references: PMID:11567029, PMID:12423684
• Mediates phagocytosis of C. albicans conidia. Related references: PMID:17230442
• Activates downstream signaling via SYK recruitment and ITAM motif, leading to NF-kB and MAP kinase p38 pathways, enhancing pro-inflammatory cytokine production in macrophages and dendritic cells (By similarity).
• Induces reactive oxygen species production and T-cell proliferation; binds T-cells in a glycan-independent manner to modulate immune responses (By similarity).
• Alternative splicing generates isoforms with varied molecular weights, reflected in observed protein weights of 5 kDa, 8 kDa, and 13-27 kDa.
• Predominantly localizes to the cell membrane and cytoplasm; highly expressed in peripheral blood leukocytes, dendritic cells, spleen, bone marrow, lung, muscle, stomach, and placenta.
• Contains an extracellular C-type lectin domain, a transmembrane helix, and an ITAM signaling motif; classified as a lectin, immune receptor, glycoprotein, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen (aa 66-247) targets the extracellular domain of CD369/Dectin-1, making it suitable for surface staining in live-cell flow cytometry. Validate staining on human myeloid cell lines (e.g., THP-1, U937) or primary monocytes/dendritic cells.
• conjugate provides direct detection without secondary antibody. Use standard PE-Texas Red filter set (excitation 590 nm, emission 617 nm). Ensure proper compensation when multiplexing with other fluorochromes.
• Multiple protein weights (5-27 kDa) due to glycosylation and isoforms may complicate western blot applications. This antibody is validated for flow cytometry; performance in other assays has not been assessed—experimental validation is recommended if used outside FC.
• Shipment on ice bag ensures product stability; upon receipt, store at -20°C as typical for monoclonal antibodies. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted B2B partner providing diagnostic manufacturers and researchers with one-stop access to high-quality IVD raw materials. This rabbit anti-human CD369/Dectin-1 monoclonal antibody exemplifies our commitment to sourcing validated immunoreagents for innate immunity and infectious disease research. We supply antibody pairs, bulk ancillary reagents, and customized procurement solutions to streamline your assay development from concept to clinic.