Rabbit anti-Human CD369/Dectin-1 mAb for FC - Q9BXN2

Core Product Specifications and Parameters

Biological Background: CD369/Dectin-1 Function and Localization

• CD369/Dectin-1 serves as a pattern recognition receptor (PRR) that specifically binds beta-1,3-linked and beta-1,6-linked glucans found in the cell walls of pathogenic bacteria and fungi. Related references: PMID:11567029 PMID:12423684
• Upon engaging beta-glucans, it mediates the phagocytosis of fungal pathogens, including Candida albicans conidia. Related references: PMID:17230442
• It signals through its intracellular ITAM motif, recruiting SYK kinase to trigger downstream NF-kappa-B and MAP kinase pathways, ultimately inducing pro-inflammatory cytokine expression.
• The receptor promotes reactive oxygen species production and enhances cytokine secretion in macrophages and dendritic cells.
• Beyond innate immunity, CD369/Dectin-1 modulates T-cell responses by binding T-cells in a glycan-independent manner, stimulating their proliferation.
• Encoded by the CLEC7A gene, alternative splicing yields multiple isoforms (observed protein weights ~5 kDa, 8 kDa, and 13–27 kDa), while the full-length polypeptide has a predicted mass of approximately 27.7 kDa and undergoes heavy glycosylation.
• Subcellular localization includes the plasma membrane and cytoplasmic compartments.
• Tissue distribution is broad but highest in peripheral blood leukocytes, dendritic cells, spleen, bone marrow, lung, muscle, stomach, and placenta.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 66–247), making this conjugate well-suited for detecting cell-surface Dectin-1 by flow cytometry.
• For optimal FC staining, titrate the antibody and validate it in your specific human immune cell population, as expression levels vary among subsets.
• If performing western blotting, anticipate multiple bands or diffuse signals due to extensive N-glycosylation; deglycosylation may be required to resolve the core protein.
• Always include appropriate isotype controls and secondary antibody controls to ensure specificity in flow cytometry experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. Our offerings include validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for innate immunity targets like CD369/Dectin-1. Whether you need rare-target reagents or high-quality conjugated antibodies for flow cytometry, CamelBio streamlines your procurement from concept to clinic.