Rabbit anti-Human CD352/SLAMF6 Monoclonal Antibody for FC - Q96DU3

Core Product Specifications and Parameters

Biological Background: SLAMF6 Function and Localization

• SLAMF6 (SLAM family member 6), also designated NTB-A (NK-T-B-antigen), CD352, Ly108, and KALI, is a self-ligand receptor belonging to the signaling lymphocytic activation molecule (SLAM) family.

• SLAM receptors, including SLAMF6, modulate the activation and differentiation of a broad range of immune cells through homo- or heterotypic cell-cell interactions, thereby connecting innate and adaptive immune responses.

• In natural killer (NK) cells, SLAMF6 engagement triggers cytolytic activity only when NK cells express high surface densities of natural cytotoxicity receptors; this positive signaling involves phosphorylation of VAV1 and depends on the adapter protein SH2D1B/EAT-2 rather than SH2D1A/SAP. Related references: PMID:11489943 PMID:16920955

• SLAMF6 promotes T-cell differentiation into a helper T-cell Th17 phenotype, leading to increased IL-17 secretion; this costimulatory activity requires SH2D1A/SAP and involves recruitment of the transcription factor RORC to the IL-17 promoter. Related references: PMID:16920955 PMID:22184727 PMID:22989874

• In the absence of adapter SH2D1A/SAP, SLAMF6 can transmit negative signals to CD4(+) T-cells and NKT cells, and may negatively regulate germinal center formation by inhibiting T-cell:B-cell adhesion, potentially contributing to the prevention of autoimmunity (By similarity).

• SLAMF6 localizes to the cell membrane and is expressed on all resting and activated NK cells, T-lymphocytes, and B-lymphocytes; increased surface expression has been reported on T-cells from systemic lupus erythematosus (SLE) patients. Related references: PMID:11489943 PMID:22184727

• The UniProt entry for SLAMF6 (Q96DU3) lists keywords including alternative splicing, glycoprotein, and disulfide bond, consistent with the reported multiple protein weights (24 kDa and 37 kDa) and the presence of immunoglobulin domains.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 24-215 of human SLAMF6, which corresponds to the extracellular region containing two Ig-like domains; thus, the antibody likely recognizes an extracellular epitope, making it well-suited for flow cytometry (FC) applications. Researchers should validate the antibody in their specific sample system, using appropriate controls such as human peripheral blood mononuclear cells (PBMCs) known to express SLAMF6.
• For FC, the antibody is directly conjugated to , a photostable fluorescent dye; consider using a standard staining protocol with appropriate cell fixation and permeabilization if intracellular epitopes are targeted. Given the expression profile on NK, T, and B cells, co-staining with lineage markers can help discriminate SLAMF6-positive populations.
• Since the antibody is validated for human reactivity, cross-reactivity with other species is not expected; users should confirm reactivity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research laboratories, and institutes from concept to clinical application. This anti-SLAMF6 antibody targets a key immune regulatory receptor relevant to autoimmune and inflammatory disease research. Whether you require validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or assistance sourcing rare targets, CamelBio offers comprehensive supply solutions to accelerate your diagnostic development.