Rabbit anti-Human CD35 Monoclonal Antibody for Flow Cytometry - P17927

Core Product Specifications and Parameters

Biological Background: CD35 Function and Localization

• CD35, also known as Complement Receptor Type 1 (CR1) or C3b/C4b receptor, is a large transmembrane glycoprotein belonging to the regulators of complement activation (RCA) family, characterized by multiple short consensus repeats (SCRs or Sushi domains).
• It functions as a membrane immune adherence receptor, mediating the capture and clearance of complement-opsonized pathogens and immune complexes by erythrocytes and monocytes/macrophages. Related references: PMID:2963069
• CD35 inhibits spontaneous complement activation by impairing the formation and function of alternative and classical pathway C3/C5 convertases, and acts as a cofactor for factor I-mediated cleavage of C3b and C4b. Related references: PMID:2972794, PMID:8175757
• Upon ligand binding, CD35 contributes to immune regulation by promoting the generation of regulatory T cells from activated helper T cells. Related references: PMID:25742728
• Additionally, CD35 serves as a receptor for Epstein-Barr virus during infection.
• The protein is expressed on the membrane of various cell types, including erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes, and granulocytes.
• CD35 features multiple Sushi (SCR) domains, a transmembrane helix, and is subject to N-linked glycosylation; it carries the Knops blood group system antigens.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a bright and photostable fluorophore suitable for direct immunofluorescence detection in flow cytometry. Consider titrating the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• As CD35 is expressed on erythrocytes and various leukocyte subsets, include proper isotype controls and Fc receptor blocking steps to minimize non-specific binding, especially in whole blood or PBMC samples.
• The immunogen corresponds to amino acids 901–1095 within the extracellular domain, which may detect a subset of CD35 isoforms or fragments; confirm reactivity with your target cell population by parallel staining with an established CD35 clone if necessary.
• For intracellular staining, note that CD35 is a transmembrane protein; permeabilization may alter epitope accessibility. Validate in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, covering complement cascade-related antibodies and reagents. For projects involving immune complex clearance, autoimmune diagnostics, or complement pathway assays, we can source validated antibody pairs, bulk ancillary reagents, and rare-target monoclonal antibodies to support your development pipeline.