Rabbit anti-Human CD35 Monoclonal Antibody for FC - P17927

Core Product Specifications and Parameters

Biological Background: CD35 Function and Localization

• Complement receptor type 1 (CR1/C3b/C4b receptor) is a membrane-bound glycoprotein that functions as a central mediator of immune adherence, capturing complement-opsonized pathogens and immune complexes for clearance by erythrocytes and phagocytes. Related references: PMID:2963069
• CR1 inhibits spontaneous complement activation by impairing the assembly and accelerating the decay of both alternative and classical pathway C3/C5 convertases. Related references: PMID:2972794; PMID:8175757
• It serves as an essential cofactor for the factor I-mediated proteolytic cleavage of C3b (to iC3b, C3c, and C3d,g) and C4b (to C4c and C4d), thereby downregulating complement cascades. Related references: PMID:2972794; PMID:8175757
• CR1 contributes to adaptive immune regulation by promoting the differentiation of regulatory T cells from activated helper T cells. Related references: PMID:25742728
• As a receptor for Epstein-Barr virus, CR1 facilitates host cell entry by the pathogen.
• CR1 is expressed on the surface of erythrocytes, B cells, follicular dendritic cells, monocytes, granulocytes, and a subset of T cells, consistent with its broad role in immune surveillance.
• The protein undergoes extensive post-translational modification, including glycosylation and disulfide bond formation, which are critical for its structural stability and ligand-binding capacity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the extracellular domain of CD35 (aa 901–1095), suggesting that the antibody may recognize epitopes accessible on live cells without permeabilization.
• This antibody is pre-conjugated to , enabling direct flow cytometry and minimizing non-specific binding associated with secondary reagents.
• For optimal staining, consider using fresh peripheral blood cells known to express CD35 (e.g., erythrocytes, B cells, monocytes) as positive controls, and titrate the antibody to determine the ideal working dilution.
• If intracellular detection is required, employ a suitable permeabilization protocol, as CD35 is a transmembrane protein with potential intracellular pools.
• Validate staining specificity using appropriate isotype controls and, if possible, CD35 gene-edited or depletion models to confirm signal authenticity.
• Avoid repeated freeze–thaw cycles and store the conjugated antibody protected from light to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a comprehensive selection of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for streamlined assay development. For complement biology and immune regulation research, we offer reliable access to CD35/CR1-targeting reagents and related pathway components, supporting projects from early-stage discovery to clinical application. Partner with CamelBio to bridge your sourcing needs, backed by dedicated technical consultation across every stage of product development.