Rabbit anti-Human CD35 mAb for FC - P17927

Core Product Specifications and Parameters

Biological Background: CD35 Function and Localization

• Complement receptor type 1 (CR1/CD35) is the C3b/C4b receptor that mediates immune adherence and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages. Related references: PMID:2963069
• CR1 inhibits spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases. Related references: PMID:2972794, PMID:8175757
• It serves as a cofactor for factor I-mediated cleavage of C3b to iC3b, C3c, and C3d,g, and of C4b to C4c and C4d, downregulating complement cascades. Related references: PMID:2972794
• Upon ligand binding, CD35 contributes to the generation of regulatory T cells from activated helper T cells, linking innate and adaptive immunity. Related references: PMID:25742728
• CR1 is a single-pass type I membrane protein and is expressed on erythrocytes, subsets of T cells, mature B cells, follicular dendritic cells, monocytes, and granulocytes.
• It contains multiple short consensus repeats (Sushi domains) and is heavily glycosylated with an apparent molecular weight of ~223kDa.
• CD35 also functions as a receptor for Epstein-Barr virus during viral entry.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 901–1095 of human CD35, a region within the extracellular domain; this antibody may detect native and recombinant CR1 in flow cytometry.
• For flow cytometry, consider using fresh whole blood or isolated PBMCs and including appropriate isotype controls and Fc receptor blocking steps.
• Validation of the antibody in the relevant sample system is recommended; optimal working concentrations should be determined empirically by titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines sourcing for diagnostic manufacturers and researchers by offering a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For complement component targets like CD35, we facilitate access to rare-target raw materials and provide technical consultation to support assay development. Whatever your immunodetection needs, CamelBio serves as a single procurement bridge from concept to clinic.