Rabbit anti-Human CD34 Monoclonal Antibody for IF/ICC and FC - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a hematopoietic progenitor cell antigen that functions as a possible adhesion molecule in early hematopoiesis, mediating stem cell attachment to the bone marrow extracellular matrix or stromal cells.
• The protein may act as a scaffold for lineage-specific glycans, enabling stem cells to bind lectins on stromal cells or other marrow components.
• CD34 presents carbohydrate ligands to selectins, facilitating cell-cell interactions.
• It is a single-pass transmembrane glycoprotein localized to the cell membrane.
• CD34 is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of various tissues.
• The protein undergoes alternative splicing, yielding isoforms with observed molecular weights of approximately 35 and 41 kDa, differing from the predicted 40.7 kDa due to glycosylation.
• Post-translational modifications include phosphorylation and glycosylation, which are critical for its function.
• Keywords: Alternative splicing, Cell adhesion, Direct protein sequencing, Glycoprotein, Membrane, Phosphoprotein, Proteomics identification, Reference proteome, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The antibody is conjugated to , a bright and photostable fluorophore, ready-to-use for immunofluorescence (IF/ICC) and flow cytometry (FC) without secondary antibody amplification.
• The immunogen corresponds to amino acids 170-290 within the extracellular domain, making it suitable for detecting surface CD34 without cell permeabilization; for intracellular detection, a fixation/permeabilization step is recommended.
• For flow cytometry, titrate the antibody to determine optimal working dilution for your specific cell type; use appropriate isotype controls.
• As CD34 is a stem cell marker, include positive controls such as human hematopoietic progenitor cells and negative controls like lymphocytes.
• Confirm cross-reactivity with human samples; reactivity with other species is not tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. Whether you need optimized monoclonal/polyclonal antibodies, specialized conjugates like this anti-CD34 mAb, or support in rare target raw-material procurement, our one-stop platform streamlines your path from concept to clinic. Trust CamelBio to accelerate your diagnostic development with reliable, research-grade materials.