Rabbit anti-Human CD34 Monoclonal Antibody for FC - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34, also known as hematopoietic progenitor cell antigen CD34, is a single-pass type I membrane protein that belongs to the CD34 family. It undergoes alternative splicing, giving rise to isoforms with apparent molecular weights of approximately 35 kDa and 41 kDa.
• It functions as a cell adhesion molecule, playing a critical role in early hematopoiesis by mediating the attachment of hematopoietic stem/progenitor cells to the bone marrow extracellular matrix and stromal cells.
• CD34 may act as a scaffold that presents lineage‑specific glycans, enabling stem cells to bind lectins expressed by stromal cells or other marrow components, thereby regulating cell localization and retention.
• It presents carbohydrate ligands to selectins, which further supports cell adhesion and trafficking within the hematopoietic niche.
• The protein is selectively expressed on the surface of hematopoietic progenitor cells and on the small vessel endothelium of various tissues, making it a key marker for stem cell isolation and vascular biology studies.
• Subcellular localization is primarily at the plasma membrane, consistent with its role in cell‑cell and cell‑matrix interactions.
• Post‑translational modifications include extensive N‑ and O‑linked glycosylation, phosphorylation, and the presence of a signal peptide and transmembrane domain.

Experimental Guidance and Technical Tips

• This antibody is designed for flow cytometry (FC) and is directly conjugated to , a violet‑laser excitable fluorophore; no secondary detection reagent is required, reducing experimental steps and background.
• The immunogen spans amino acids 170–290 of human CD34 (within the extracellular region), suggesting that the antibody recognizes an extracellular epitope on live cells; consider gentle cell handling to preserve surface antigen integrity.
• For FC applications, titrate the antibody to determine the optimal concentration for your cell type and staining conditions, and include appropriate isotype controls and fluorescence minus one (FMO) controls.
• Validate performance with known CD34‑positive human cells (e.g., KG‑1a or MOLM‑13 cell lines) or primary hematopoietic progenitor cells, and consider co‑staining with other stem cell markers to assess progenitor subpopulations.
• The predicted molecular weights of 35 kDa and 41 kDa may correspond to differently glycosylated isoforms; this information can be used to confirm specificity in western blotting if the antibody is validated for that application, but primary recommendation remains FC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering bulk‑validated monoclonal antibodies, optimized antibody pairs, and ancillary reagents to diagnostic manufacturers and research laboratories. With a focus on stem cell and hematopoiesis research, we help streamline your supply chain for CD34 detection reagents and related targets, from concept to clinical application. Our one‑stop platform bridges the gap between demand and rare‑target sourcing, ensuring you have the critical raw materials needed for your assay development and clinical translation.