Rabbit anti-Human CD337/NKp30 Monoclonal Antibody for IF/ICC and FC (intra) - O14931

Core Product Specifications and Parameters

Biological Background: CD337/NKp30 Function and Localization

• The protein natural cytotoxicity triggering receptor 3 (NCR3), also known as NKp30 or CD337, is a receptor on NK cells that belongs to the immunoglobulin superfamily. It exists as multiple isoforms due to alternative splicing, resulting in protein weights ranging from ~16 to 21 kDa (observed) with a theoretical molecular weight of ~21.6 kDa.
• NCR3 acts as an activating receptor on natural killer (NK) cells, stimulating cytotoxicity upon binding to extracellular ligands such as BAG6 and NCR3LG1.
• Engagement of NCR3 by BAG6 promotes myeloid dendritic cell (DC) maturation, both by killing immature DCs and by inducing NK cells to release TNF-α and IFN-γ, which in turn drive DC maturation.
• The receptor is selectively expressed by all resting and activated NK cells, with weak expression also detected in the spleen.
• Subcellularly, NCR3 localizes to the cell membrane, consistent with its role as a transmembrane receptor.
• The protein features an immunoglobulin domain, a signal peptide, a transmembrane helix, and disulfide bonds; it is also glycosylated. Keywords from UniProt include alternative splicing, glycoprotein, and immunity.

Experimental Guidance and Technical Tips

• Given that the immunogen corresponds to the extracellular domain (amino acids 19-135), the antibody may detect surface-expressed NCR3 without permeabilization. For intracellular flow cytometry, permeabilization is required; optimize fixation and permeabilization protocols for NK cell lines.
• The observed protein weight range (16–21 kDa) suggests multiple isoforms or post‑translational modifications; validate specificity in your cell system using appropriate controls.
• As NCR3 is selectively expressed on NK cells, include control cell types (e.g., T cells) to confirm specificity in IF/ICC experiments.
• For IF/ICC, test a range of antibody dilutions on paraformaldehyde‑fixed cells; antigen retrieval may improve signal if epitope masking is suspected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti‑CD337 clone for NK cell receptor studies. Our supply network covers bulk ancillary reagents and rare target sourcing to support your assay development from R&D to clinical application. With expertise spanning the entire IVD pipeline, CamelBio is your trusted partner for reliable, high‑quality raw materials and technical support.