Rabbit anti-Human CD337/NKp30 Monoclonal Antibody for Flow Cytometry - O14931

Core Product Specifications and Parameters

Biological Background: CD337/NKp30 Function and Localization

• CD337, also known as NKp30 or NCR3, is a natural cytotoxicity triggering receptor 3 expressed on natural killer (NK) cells. It is a type I transmembrane protein with a single immunoglobulin-like domain in its extracellular region.
• NKp30 plays a critical role in NK cell-mediated cytotoxicity. Upon ligand binding, it stimulates NK cells to kill target cells, including tumor cells, through the release of cytotoxic granules.
• The known ligands for NKp30 include BAG6 (HLA-B-associated transcript 3) and NCR3LG1 (B7-H6). Engagement by BAG6 not only triggers NK cytotoxicity but also promotes maturation of myeloid dendritic cells (DCs) via NK cell-derived cytokines.
• The protein exists in multiple isoforms due to alternative splicing, which may modulate receptor function and signaling.
• Subcellularly, NKp30 localizes to the cell membrane, consistent with its role as a cell surface receptor.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are important for proper folding and ligand recognition.
• NKp30 is selectively expressed on resting and activated NK cells, with very low expression detected in the spleen.
• As a member of the immunoglobulin superfamily, NKp30 contains a signal peptide, an extracellular Ig-like domain, a transmembrane helix, and a short cytoplasmic tail, lacking intrinsic signaling motifs but associating with adaptor molecules like CD3ζ and FcεRIγ to transduce activation signals.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 19-135) of human NKp30, suggesting that the antibody likely recognizes an epitope accessible on the cell surface.
• This conjugate is designed for direct immunofluorescence detection in flow cytometry. For optimal staining, perform antibody titration using a relevant cell line (e.g., NK92) or primary human NK cells.
• Include appropriate compensation controls when using this antibody in multicolor panels, as may have spectral overlap with other fluorophores.
• Given the low molecular weight prediction (~21 kDa) and possible glycosylation, the protein may appear as a diffuse band in western blotting; however, its parent clone's suitability for WB must be verified independently.

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