Rabbit Anti-Human CD337/NKp30 Monoclonal Antibody for Flow Cytometry - O14931

Core Product Specifications and Parameters

Biological Background: CD337/NKp30 Function and Localization

• CD337/NKp30, encoded by the NCR3 gene, is a natural cytotoxicity triggering receptor 3, also known as activating natural killer receptor p30 (NKp30) or NK cell p30-related protein.
• Functions as a cell membrane receptor on natural killer (NK) cells, activated by extracellular ligands such as BAG6 and NCR3LG1, to stimulate NK cell cytotoxicity against target cells expressing these ligands.
• Plays a critical role in tumor immune surveillance by controlling NK cell-mediated killing of tumor cells.
• Engagement of NCR3 by BAG6 promotes myeloid dendritic cell (DC) maturation via two mechanisms: direct killing of immature DCs and induction of TNFA and IFNG release by NK cells, which further drives DC maturation.
• Localizes to the cell membrane.
• Selectively expressed on all resting and activated NK cells, with weak expression in the spleen.
• Key structural features include an immunoglobulin domain, signal peptide, transmembrane helix, disulfide bonds, and glycosylation; also subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 19–135) of human CD337/NKp30, making this antibody well-suited for detecting surface-expressed receptor on live cells via flow cytometry.
• For optimal staining, consider titrating the antibody on relevant NK cell lines (e.g., NK-92) or primary human NK cells; use an appropriate isotype control to assess background.
• Because CD337/NKp30 undergoes glycosylation and alternative splicing, verify specificity by comparing signals in NCR3-positive versus NCR3-knockdown or -knockout models.
• For multicolor flow cytometry panels, confirm compatibility of this rabbit monoclonal antibody with secondary detection reagents or directly conjugated formats, and proceed with appropriate Fc receptor blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This rabbit anti-human CD337/NKp30 monoclonal antibody supports oncology and immuno-oncology research, enabling detection of NK cell activity. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to meet your development and manufacturing needs.