Rabbit anti-Human CD335/NKp46 Monoclonal Antibody for IF/ICC, FC - O76036

Core Product Specifications and Parameters

Biological Background: CD335/NKp46 Function and Localization

• NCR1 (CD335/NKp46) is a natural cytotoxicity triggering receptor selectively expressed on natural killer (NK) cells.
• It functions as an activating receptor that enhances the efficiency of NK cell-mediated tumor cell lysis.
• The protein belongs to the immunoglobulin superfamily and contains immunoglobulin-like domains, characteristic of NK cell receptors.
• It is expressed by both resting and activated NK cells, underscoring its role in innate immune surveillance.
• The receptor localizes to the cell membrane, with a transmembrane domain and signal peptide for cell surface expression.
• Multiple isoforms are generated by alternative splicing, yielding predicted molecular weights from 21 to 34 kDa.
• Post-translational modifications include glycosylation and intramolecular disulfide bonds, which contribute to structural stability.
• As an NK cell-activating receptor, it is involved in recognizing and eliminating virus-infected and tumor cells.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 22-254) and is expected to recognize native surface-expressed NKp46. Verify reactivity in resting and activated NK cell lines or primary cells.
• For flow cytometry (FC), titrate the antibody to determine optimal staining concentration and include appropriate isotype control. The conjugate is compatible with standard red laser (633–647 nm) detection channels.
• For immunofluorescence/immunocytochemistry (IF/ICC), use cell surface staining protocols with live, unfixed cells or mild fixation that preserves epitope integrity. Confirm specificity using NKp46-negative cell lines.
• Consider multiplexing with other markers (e.g., CD56, CD16) to characterize NK cell subsets. Validate co-staining in the relevant experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers rapid access to this conjugate and the complete CD335/NKp46 antibody portfolio, supporting natural killer cell research and diagnostic assay development. Our supply chain covers validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets—helping you transition smoothly from concept to clinic. Partner with CamelBio to streamline procurement and accelerate your IVD initiatives.