Rabbit anti-Human CD335/NKp46 mAb - O76036

Core Product Specifications and Parameters

Biological Background: CD335/NKp46 Function and Localization

• CD335/NKp46 is a natural cytotoxicity triggering receptor encoded by the NCR1 gene, belonging to the immunoglobulin superfamily.
• It functions as a cytotoxicity-activating receptor that enhances the ability of activated natural killer (NK) cells to mediate tumor cell lysis.
• The protein is selectively expressed on the surface of both resting and activated NK cells.
• CD335/NKp46 contains two immunoglobulin-like domains and is anchored to the cell membrane via a transmembrane helix.
• Alternative splicing produces multiple isoforms, resulting in predicted molecular weights ranging from 21 to 34 kDa.
• Post-translational modifications include a cleaved signal peptide, disulfide bonds, and glycosylation sites, which are important for its structural integrity and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–254), making this antibody suitable for detection of surface CD335/NKp46 by immunofluorescence and flow cytometry.
• For flow cytometry, consider using a live-cell staining protocol to preserve cell surface epitopes and include appropriate isotype controls to confirm specificity.
• Validate the antibody in your experimental system by testing on NK cell lines or isolated primary NK cells, as CD335/NKp46 is a selective NK cell marker.
• Optimize antibody dilution for your assay; typical starting concentrations for flow cytometry range from 0.5 to 5 µg/mL, but titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive procurement solution for IVD raw materials, serving diagnostic manufacturers and research institutions with end-to-end support. This anti-CD335/NKp46 antibody supports NK cell research and tumor immunity studies, aligning with our extensive portfolio of validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For rare target raw-material sourcing or custom development, CamelBio provides tailored supply capabilities to streamline your workflow from concept to clinic.