Rabbit anti-Human CD33 Monoclonal Antibody for Flow Cytometry - P20138

Core Product Specifications and Parameters

Biological Background: CD33 Function and Localization

• Myeloid cell surface antigen CD33 (Siglec-3, gp67) is a single-pass type I membrane protein belonging to the sialic acid-binding immunoglobulin-like lectin (Siglec) family (UniProt P20138).
• CD33 functions as a sialic acid-binding receptor that preferentially recognizes α2,3- and α2,6-linked sialic acid-bearing glycans (PubMed:7718872). Related references: PMID:7718872
• The cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs); upon ligand engagement such as C1q or sialylated glycoproteins, ITIMs are phosphorylated by Src-like kinases (e.g., LCK) (PubMed:10887109, PubMed:28325905). Related references: PMID:10887109 , PMID:28325905
• Phosphorylated ITIMs recruit protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, which dephosphorylate downstream signaling molecules to maintain immune cells in a resting state (PubMed:10206955, PubMed:10556798, PubMed:10887109). Related references: PMID:10206955 , PMID:10556798 , PMID:10887109
• CD33-mediated repression in monocytes also requires phosphoinositide 3-kinase/PI3K activity (PubMed:15597323). Related references: PMID:15597323
• Subcellular localization includes the plasma membrane and peroxisomes; tissue expression is primarily in monocytic/myeloid lineage cells and microglia in the brain.
• The protein undergoes alternative splicing and post-translational modifications including glycosylation and phosphorylation (UniProt Keywords).

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (a.a. 18-259) of human CD33; therefore, the antibody may recognize the native conformation of the surface antigen and is expected to be suitable for flow cytometry applications.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your cell system (e.g., human PBMCs or myeloid cell lines).
• Validate antibody performance in the relevant sample type and application, as performance may vary with fixation and permeabilization protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, technical services, and consulting support. For CD33-targeted studies—such as immune cell typing, cancer immunophenotyping, or Alzheimer's disease research—CamelBio can serve as your sourcing partner for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. Whether you need research-grade reagents or scalable raw materials for assay development, CamelBio aims to streamline your supply chain from concept to clinic.