Rabbit anti-Human CD326/EPCAM Monoclonal Antibody for IF/ICC and FC - P16422

Core Product Specifications and Parameters

Biological Background: Epithelial Cell Adhesion Molecule (EpCAM) Function and Localization

• EpCAM is a type I transmembrane glycoprotein that functions as a homophilic cell adhesion molecule, mediating physical interactions between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) to maintain the mucosal immunological barrier.
• It plays a critical role in embryonic stem cell proliferation and differentiation, with expression levels rapidly declining upon differentiation.
• EpCAM up-regulates the expression of FABP5, MYC, and cyclins A and E, linking it to cell cycle regulation and growth control.
• The protein is localized at the lateral cell membrane and tight junctions, consistent with its role in cell-cell adhesion and epithelial integrity.
• Tissue specificity: Highly and selectively expressed in undifferentiated embryonic stem cells and almost all epithelial cell membranes, but absent from mesodermal or neural cell membranes; frequently found on the surface of adenocarcinomas.
• It is a well-characterized tumor antigen and is associated with hereditary nonpolyposis colorectal cancer.
• The processed protein contains a signal peptide, transmembrane helix, disulfide bonds, and N-glycosylation sites, with a calculated molecular mass of approximately 34.9 kDa.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 24-265), which is expected to generate antibodies targeting accessible epitopes on the cell surface, suitable for live-cell staining or non-permeabilized conditions.
• For immunofluorescence (IF/ICC), consider optimizing fixation and permeabilization protocols based on target localization at lateral membranes and tight junctions; validate in human epithelial cell lines.
• In flow cytometry (FC), use the directly conjugated format; titrate the antibody to achieve optimal signal-to-noise ratio and include appropriate isotype controls.
• Due to human cross-reactivity only, verify target expression in your cell or tissue model before use.
• Protein weight is approximately 35 kDa; confirm with molecular weight markers and blocking conditions in downstream assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a comprehensive portfolio that includes this Rabbit anti-Human CD326/EPCAM mAb for immunology and oncology research. We support diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing of rare target raw materials—helping you bridge from concept to clinic.