Rabbit anti-Human CD326/EPCAM Monoclonal Antibody for FC - P16422

Core Product Specifications and Parameters

Biological Background: Epithelial cell adhesion molecule Function and Localization

• Epithelial cell adhesion molecule (EPCAM), also designated CD326, is a type I transmembrane glycoprotein and a member of the tumor-associated calcium signal transducer family.
• It mediates homophilic cell adhesion between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium, providing an immunological barrier against mucosal infection.
• EPCAM plays a role in embryonic stem cell proliferation and differentiation, up-regulating the expression of FABP5, MYC, and cyclins A and E.
• The protein localizes to the lateral cell membrane and tight junctions, consistent with its adhesive function.
• It is highly expressed in undifferentiated embryonic stem cells, with levels rapidly diminishing upon differentiation; it is also present on almost all epithelial cell membranes, but absent from mesodermal and neural cell membranes.
• EPCAM is frequently overexpressed on the surface of adenocarcinomas, classifying it as a tumor antigen and a target for cancer diagnostics and therapeutics.
• Post-translational modifications include disulfide bond formation and glycosylation, and the protein contains a signal peptide and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 24–265), suggesting the antibody targets an epitope accessible on the cell surface, suitable for flow cytometry without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your experimental system, and include appropriate isotype controls to assess nonspecific binding.
• Use low-speed centrifugation and gentle resuspension to maintain cell viability, as EPCAM is a surface antigen; avoid harsh enzymatic digestion that may cleave extracellular epitopes.
• Due to the fluorophore conjugation, protect samples from light and analyze promptly to minimize photobleaching.
• Validate reactivity in epithelial-derived cell lines known to express EPCAM, such as MCF-7 (breast adenocarcinoma) or HT-29 (colorectal adenocarcinoma), noting that expression levels may differ with differentiation state.
• Since EPCAM is a tumor antigen, consider gating strategies to distinguish epithelial cells from non-epithelial populations in mixed samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focused on epithelial cell adhesion, cancer biomarkers, and stem cell biology, this anti-CD326/EPCAM monoclonal antibody exemplifies CamelBio’s ability to supply validated antibodies, bulk ancillary reagents, and rare target raw materials. Let CamelBio streamline your IVD development from concept to clinic.