Rabbit anti-Human CD31/PECAM1 Monoclonal Antibody for Flow Cytometry - P16284

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• CD31, also known as platelet endothelial cell adhesion molecule (PECAM-1), endoCAM or GPIIA', is a glycoprotein member of the immunoglobulin superfamily that functions as a cell adhesion molecule.
• It is required for leukocyte transendothelial migration (TEM) during inflammation, with phosphorylation of Tyr-690 playing a critical role in TEM and intracellular trafficking.
  Related references: PMID:17580308, PMID:19342684
• PECAM-1 mediates both homophilic interactions at endothelial cell junctions and heterophilic binding with CD177 on neutrophils, facilitating cell-cell adhesion and immune cell recruitment.
  Related references: PMID:27958302, PMID:17580308
• Homophilic ligation of PECAM-1 regulates phagocytosis: it prevents macrophage engulfment of viable leukocytes while promoting clearance of apoptotic cells.
  Related references: PMID:12110892
• The protein modulates bradykinin receptor BDKRB2 activity and downstream ERK1/2 signaling in endothelial cells.
  Related references: PMID:18672896
• Subcellularly, PECAM-1 is located on the cell membrane, concentrated at membrane rafts and cell junctions. It is highly expressed on platelets, leukocytes, and endothelial cells, with isoform‑specific tissue distribution (e.g., Delta12 in trachea, Delta14‑15 in lung).
  Related references: PMID:18388311, PMID:12433657

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 28–601), making this antibody suitable for detection of cell surface CD31 by flow cytometry.
• Titrate the antibody for optimal staining of your target cell population (e.g., human PBMCs, endothelial cells, or platelets).
• Account for the conjugate in multicolor panel design; use proper single‑stain controls and consider compensation requirements.
• Include a viability marker to exclude dead cells, which may nonspecifically bind antibody conjugates.
• Consider pre‑incubating cells with Fc receptor blocking reagents to minimize background binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing of IVD raw materials, supporting diagnostic developers from concept to clinic. This anti‑CD31/PECAM1 monoclonal antibody aids flow‑cytometric studies in immunology, vascular biology, and inflammation. In addition to validated antibodies, we supply matched antibody pairs, bulk ancillary reagents, and can source rare‑target raw materials to accelerate your assay development.