Rabbit anti-Human CD31/PECAM1 mAb for FC - P16284

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• CD31/PECAM-1 is a cell adhesion molecule essential for leukocyte transendothelial migration (TEM) during inflammation. Related references: PMID:17580308 PMID:19342684
• Phosphorylation at Tyr-690 is critical for TEM, regulating PECAM-1 trafficking to and from the lateral border recycling compartment (LBRC). Related references: PMID:19342684
• Homophilic and heterophilic interactions (with CD177) mediate endothelial cell-cell adhesion and neutrophil transendothelial migration. Related references: PMID:27958302 PMID:17580308
• PECAM-1 homophilic ligation prevents phagocytosis of viable leukocytes but promotes tethering and phagocytosis of apoptotic cells. Related references: PMID:12110892
• It modulates bradykinin receptor BDKRB2 activation and ERK1/2 signaling in endothelial cells. Related references: PMID:18672896
• Subcellularly, PECAM-1 localizes to the cell membrane, membrane rafts, and cell junctions.
• Tissue expression: highly expressed on platelets, leukocytes, and concentrated at endothelial cell borders; multiple isoforms with tissue-specific distribution (e.g., Delta12 in trachea, Delta14-15 in lung).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 28–601 of human CD31, encompassing the extracellular immunoglobulin-like domains. This design may favor generation of antibodies that recognize native epitopes on live cells.
• For flow cytometry (FC), this directly conjugated antibody is suitable for surface staining of human cells. Consider titrating the antibody to determine the optimal working concentration for your specific experimental system.
• CD31 is expressed on endothelial cells, platelets, and leukocytes; include appropriate positive and negative controls (e.g., cell lines or knockout/knockdown models) to confirm specificity.
• As a monoclonal antibody, epitope accessibility might be affected by fixation/permeabilization protocols; validate in the relevant sample matrix if intracellular staining is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CD31/PECAM1-related research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our streamlined supply chain and technical support help accelerate your endothelial and leukocyte biology projects.