Rabbit anti-Human CD317/BST2 Monoclonal Antibody for FC - Q10589

Core Product Specifications and Parameters

Biological Background: CD317/BST2 Function and Localization

• Bone marrow stromal antigen 2 (BST2), also known as HM1.24 antigen or tetherin, is an interferon-induced antiviral host restriction factor. Related references: PMID:18200009 PMID:18342597 PMID:19036818

• Acts as a physical tether for nascent virions, linking them to the cell membrane or each other, thereby blocking release of diverse enveloped viruses including retroviruses, filoviruses, arenaviruses, herpesviruses, rhabdoviruses, orthomyxoviruses, paramyxoviruses, and coronaviruses. Related references: PMID:18200009 PMID:18342597 PMID:19179289

• Encodes two isoforms: isoform 1 activates NF-kappa-B and is degraded by HIV-1 Vpu, while isoform 2 is resistant to Vpu and restricts viral budding in its presence, also inhibiting isoform 1-mediated signaling. Related references: PMID:23028328 PMID:26172439

• Localizes to the Golgi apparatus, trans-Golgi network, cell membrane, membrane rafts, cytoplasm, apical cell membrane, and late endosomes.

• Predominantly expressed in liver, lung, heart, and placenta; overexpressed in multiple myeloma cells; highly expressed on B-cells, T-cells, monocytes, NK cells, and dendritic cells.

• GPI-anchored membrane protein with a signal-anchor transmembrane helix, N-glycosylation, and disulfide bonds.

• Negatively regulates matrix metalloproteinase MMP14, decreasing activation of MMP15 and inhibiting cell growth and migration. Related references: PMID:22065321

• Stimulates LILRA4/ILT7 signaling in plasmacytoid dendritic cells, providing negative feedback to IFN production during viral infection. Related references: PMID:19564354 PMID:26172439

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 49–160) of human CD317/BST2, which includes the coiled-coil region involved in dimerization and viral tethering; consider validating antibody binding to native and recombinant protein in the relevant sample system.

• Conjugated with , this antibody is suitable for flow cytometry; optimal staining concentrations should be titrated using known positive cell lines such as multiple myeloma-derived cells or IFN-treated primary cells.

• Verify specificity by comparing staining in wild-type versus BST2-knockout or -knockdown cells, and account for potential Vpu-mediated downregulation in HIV-1-infected samples.

• Complementary applications may include immunofluorescence on permeabilized cells to study subcellular localization, though performance should be experimentally confirmed.

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