Rabbit anti-Human CD317/BST2 mAb for Flow Cytometry - Q10589

Core Product Specifications and Parameters

Biological Background: CD317/BST2 Function and Localization

• Official full name: Bone marrow stromal antigen 2 (BST2), also known as HM1.24 antigen or Tetherin. Encoded by the BST2 gene.
• Acts as an interferon-induced antiviral restriction factor that directly tethers budding enveloped viruses to the host cell membrane, preventing virus release. Targets diverse viruses including HIV-1, HIV-2, SIV, Ebola, Marburg, Lassa, SARS-CoV, and influenza A. Related references: PMID:18200009 PMID:18342597 PMID:19036818
• Isoform 1 and isoform 2 have differing antiviral and signaling activities; isoform 2 is resistant to HIV-1 Vpu-mediated degradation. Related references: PMID:23028328 PMID:26172439
• Subcellular localization: found in trans-Golgi network, cell membrane, membrane rafts, cytoplasm, apical cell membrane, and late endosomes.
• Tissue specificity: predominantly expressed in liver, lung, heart, and placenta; overexpressed in multiple myeloma cells. High expression in B-cell lineage, T-cells, monocytes, NK cells, and dendritic cells.
• Post-translational modifications include glycosylation, GPI-anchor, disulfide bonds, and isopeptide bond (ubiquitin-like conjugation).
• Plays a role in B-cell activation and innate immunity; also inhibits MMP14 proteolytic activity affecting cell growth and migration. Related references: PMID:22065321
• Stimulates LILRA4/ILT7 signaling, providing negative feedback on IFN production by plasmacytoid dendritic cells. Related references: PMID:19564354 PMID:26172439

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 49-160 of human BST2, which covers the extracellular domain. This region is critical for viral tethering activity and is targeted by viral antagonists such as HIV-1 Vpu. Consider this when designing functional studies.
• This conjugate is designed for direct flow cytometry detection of CD317 on live cells. Optimize staining conditions and include appropriate isotype controls.
• Given the expression of BST2 on multiple immune cell subsets and multiple myeloma cells, validate the antibody in your specific sample system and consider using a viability dye to exclude dead cells.
• Cross-reactivity with human is reported; predict cross-reactivity with other species is unknown and should be tested empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated IVD raw materials, including optimized monoclonal antibodies like this anti-CD317/BST2 clone. As a comprehensive sourcing partner, we support the development of flow cytometry panels, infectious disease research, and immuno-oncology assays with a wide range of ancillary reagents and rare target supplies. Our technical consulting services further assist in transitioning from concept to clinical application.