Rabbit anti-Human CD317/BST2 mAb for FC - Q10589

Core Product Specifications and Parameters

Biological Background: CD317/BST2 Function and Localization

• BST2 (Bone marrow stromal antigen 2), also known as tetherin or HM1.24, is an interferon-inducible antiviral host restriction factor.
• BST2 directly tethers nascent enveloped virus particles to the infected cell membrane, preventing their release as cell-free virions. This tethering can lead to endocytosis and degradation of trapped virions. Related references: PMID:18200009, PMID:18342597, PMID:19036818
• It restricts a broad range of mammalian enveloped viruses, including HIV-1, HIV-2, SIV, EIAV, HCV, Ebola, Marburg, influenza A, and SARS-CoV. Related references: PMID:18200009, PMID:18342597, PMID:19179289
• Beyond viral restriction, BST2 inhibits the cell-surface proteolytic activity of MMP14, leading to decreased activation of MMP15 and suppression of cell growth and migration. Related references: PMID:22065321
• It mediates signaling through LILRA4/ILT7 on plasmacytoid dendritic cells, providing negative feedback to IFN production during viral infection. Related references: PMID:19564354, PMID:26172439
• Two isoforms exist: isoform 1 and isoform 2. Both restrict viral budding, but isoform 2 is resistant to HIV-1 Vpu-mediated degradation and has distinct signaling properties; isoform 1 activates NF-kappa-B, an activity inhibited by isoform 2. Related references: PMID:23028328, PMID:26172439
• Subcellularly, BST2 localizes to the trans-Golgi network, cell membrane, lipid rafts, apical membrane, late endosomes, and cytoplasm.
• Predominantly expressed in liver, lung, heart, and placenta; lower levels in pancreas, kidney, skeletal muscle, and brain. Highly expressed on B-cells, T-cells, monocytes, NK cells, and dendritic cells. Overexpressed in multiple myeloma cells.
• Key structural features include a coiled-coil domain, GPI-anchor, transmembrane helix, disulfide bonds, and N-linked glycosylation. It is a type II membrane protein with a molecular weight of ~20 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 49-160), making this antibody well-suited for detecting surface BST2 by flow cytometry. Consider titrating the antibody to determine optimal staining concentrations.
• Since BST2 expression is upregulated by type I interferons, pre-treatment of cells with IFN may enhance detection sensitivity.
• Due to the GPI-anchor, no permeabilization is required for surface staining; for intracellular detection, optimize fixation and permeabilization protocols.
• Include appropriate isotype controls and single-stain compensation controls when performing multicolor flow cytometry.
• Validate the antibody in your relevant human sample system, as expression levels can vary by cell type and activation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with reliable IVD raw materials. This -conjugated CD317/BST2 antibody supports flow cytometry applications in antiviral immunity and hematological malignancy research. Beyond single antibodies, we offer validated antibody pairs, ancillary reagents, and sourcing for rare targets to streamline your assay development.