Rabbit anti-Human CD314 (NKG2D) Monoclonal Antibody for FC - P26718

Core Product Specifications and Parameters

Biological Background: NKG2-D type II integral membrane protein Function and Localization

• Encoded by the KLRK1 gene, NKG2-D (also known as CD314, NKG2D, D12S2489E) is a type II integral membrane protein and a member of the killer cell lectin-like receptor family.
• Functions as an activating and costimulatory receptor involved in immunosurveillance by binding to stress-inducible MHC class I-related ligands (MICA, MICB, ULBP1-4, RAET1 members) on tumor or virus-infected cells.
• Upon ligand engagement, triggers calcium influx and downstream signaling cascades that stimulate cytotoxic activity and TNF expression in NK cells.
• Acts as a costimulatory receptor for the T-cell receptor in CD8+ T cells, amplifying adaptive immune responses and promoting perforin-mediated target cell lysis.
• Participates in NK cell-mediated bone marrow graft rejection and may regulate NK cell differentiation and survival.
• Localizes to the cell membrane and is expressed predominantly on NK cells (CD56+CD3-), CD8+ αβ and γδ T cells, and interferon-producing killer dendritic cells (IKDCs).
• Undergoes alternative splicing, producing multiple isoforms; the canonical form has a calculated molecular weight of approximately 25.3 kDa.
• Keywords: Adaptive immunity, Alternative splicing, Disulfide bond, Glycoprotein, Innate immunity, Lectin, Signal-anchor, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 73–216), which encompasses the ligand-binding region; this design may favor recognition of the native conformation in flow cytometry.
• For flow cytometry applications, titrate the antibody to determine the optimal staining concentration for your specific cell type, as CD314 expression levels can vary with NK and T-cell activation status.
• When designing multicolor panels, consider the spectral properties of (similar to FITC) and include appropriate compensation controls to avoid spillover.
• Store the antibody at 2–8°C and protect from light to maintain fluorophore stability; avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined, one-stop sourcing solution for IVD raw materials, including this anti-CD314 (NKG2D) monoclonal antibody for flow cytometry. Our extensive portfolio covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support immuno-oncology and immunosurveillance research. From concept to clinic, CamelBio bridges the gap, delivering high-quality raw materials with the technical support needed for your diagnostic development.