Rabbit anti-Human CD314 Monoclonal Antibody for FC - P26718

Core Product Specifications and Parameters

Biological Background: NKG2-D type II integral membrane protein Function and Localization

• NKG2-D (also known as CD314, killer cell lectin-like receptor subfamily K member 1, or KLRK1) is a C-type lectin-like transmembrane receptor that functions as a key activating and costimulatory molecule in innate and adaptive immunity.
• It recognizes stress-inducible MHC class I-related ligands (MICA, MICB, ULBP1-6, RAET1E/G/L) on tumor and virus-infected cells, triggering natural killer (NK) and CD8(+) T-cell cytotoxic responses through calcium-dependent signaling and perforin-mediated elimination.
• The receptor is encoded by the KLRK1 gene and is expressed as a type II integral membrane protein on the cell surface, where it forms disulfide-linked homodimers essential for ligand engagement and signal transduction.
• It is predominantly expressed in NK cells, CD8(+) alpha-beta and gamma-delta T-cells, and interferon-producing killer dendritic cells (IKDCs), and is found on virtually all CD56(+)CD3(-) NK cells from peripheral blood.
• In addition to its activating role, NKG2-D acts as a costimulatory receptor for the T-cell receptor (TCR) in CD8(+) T-cells, amplifying adaptive immune responses and promoting expression of TNF-alpha.
• The protein undergoes alternative splicing, N-linked glycosylation, and contains a signal-anchor transmembrane domain, contributing to its functional diversity and stability on the plasma membrane.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , enabling single-step flow cytometric detection of surface NKG2D without the need for secondary reagents; consider it for multi-color immunophenotyping panels targeting human NK and T-cell subsets.
• The immunogen corresponds to amino acids 73–216 of the extracellular domain, suggesting the antibody recognizes an epitope involved in ligand binding or dimerization; validate staining patterns on known NKG2D-positive cell lines or primary PBMCs.
• For optimal resolution, titrate the antibody under your experimental conditions and include appropriate isotype controls. Given the receptor’s stress-inducible ligand interactions, consider blocking steps if working with ligand-expressing target cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. This NKG2D/CD314 antibody serves immuno-oncology and immune profiling applications, and we offer a broad portfolio of validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare or emerging targets. Whether you need off-the-shelf flow cytometry antibodies or tailored raw-material supply for assay development, CamelBio is your dedicated partner in translational research and in vitro diagnostics.