Rabbit anti-Human CD307b Monoclonal Antibody for Flow Cytometry - Q96LA5

Core Product Specifications and Parameters

Biological Background: CD307b (FCRL2) Function and Localization

• CD307b, also known as Fc receptor-like protein 2 (FCRL2), FcRH2, or IRTA4, is a transmembrane receptor belonging to the immunoglobulin superfamily. It contains multiple immunoglobulin-like domains and is part of the Fc receptor-like family.
• The protein may play a regulatory role in normal and neoplastic B cell development, as suggested by its expression pattern and structural features.
• FCRL2 is encoded by the FCRL2 gene in humans and is located on chromosome 1q23.2. It undergoes alternative splicing, producing multiple isoforms with distinct tissue distributions.
• Subcellularly, CD307b is anchored at the cell membrane via a single transmembrane helix, with an extracellular domain containing potential glycosylation sites and disulfide bonds that stabilize its immunoglobulin folds.
• Tissue expression is restricted to mature B-cell lines, particularly in secondary lymphoid organs such as the spleen and lymph nodes. It is highly expressed in CD19+ B cells and within the mantle zones of tonsil tissue. At the protein level, it is preferentially detected in memory B cells.
• Isoform 2 is expressed in the spleen, peripheral blood, and bone marrow, while isoforms 2 and 4 are present in B-cell lines, indicating a complex regulation of expression.
• Post-translational modifications include glycosylation as a glycoprotein, and the protein contains conserved disulfide bonds important for its domain architecture. These features are typical of cell surface receptors involved in immune signaling.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fusion protein spanning amino acids 20–395 of human CD307b, which corresponds to the entire extracellular domain (ECD). This design likely produces antibodies that recognize the native conformation of the cell surface receptor.
• As this antibody is conjugated to , it is directly ready for flow cytometry (FC) without the need for secondary staining. Consider titrating the antibody to determine the optimal working concentration for your specific B-cell samples.
• Given the restricted expression of CD307b on mature and memory B cells, include appropriate positive controls (e.g., human peripheral blood mononuclear cells or tonsil-derived B cells) and isotype controls to ensure specificity.
• Because the antibody is validated for human reactivity only, cross-reactivity with other species is not expected. If using in a non-human system, pre-testing is advised if cross-reactivity is suspected.
• For FC staining, ensure that cells are viable and that Fc receptors are blocked to minimize non-specific binding, especially when working with B-cell populations that express other Fc receptors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research institutions, providing high-quality raw materials tailored to B-cell immunology and hematologic malignancy research. This anti-CD307b monoclonal antibody exemplifies our capability to supply validated detection reagents, including conjugated antibodies for direct flow cytometry applications, to accelerate your diagnostic development. Beyond single antibodies, we offer matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare target raw materials, streamlining your journey from concept to clinical application.