Rabbit anti-Human CD307b (FCRL2) Monoclonal Antibody for FC - Q96LA5

Core Product Specifications and Parameters

Biological Background: CD307b/FCRL2 Function and Localization

• Fc receptor-like protein 2 (FCRL2, also known as CD307b, FcRH2, IRTA4, SPAP1) is a member of the immunoglobulin receptor superfamily; it contains immunoglobulin domains, a transmembrane helix, and an intracellular signaling motif, functioning as a putative receptor.
• The FCRL2 gene (alias FCRH2, IFGP4, IRTA4, SPAP1) encodes multiple isoforms via alternative splicing; these isoforms show tissue-specific expression, with isoform 2 and 4 expressed in B-cell lines.
• CD307b may have a regulatory role in normal and neoplastic B cell development, potentially modulating B cell differentiation and immune responses.
• The protein localizes to the cell membrane, consistent with its role as a cell surface receptor on B cells.
• Tissue expression is restricted to mature B-cell populations in secondary lymphoid organs (spleen, lymph node); it is highly expressed in CD19+ B cells and within mantle zones of tonsil tissue.
• At the protein level, CD307b is preferentially expressed in memory B cells, suggesting involvement in long-term humoral immunity.
• Post-translational modifications include glycosylation and disulfide bond formation; the predicted molecular weight is approximately 55.5 kDa, with electrophoretic migration at ~56 kDa.
• Additional annotated keywords: Alternative splicing, Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fusion protein covering amino acids 20–395 of human CD307b, NP_110391.2) represents the extracellular domain, so the resulting antibody can likely bind native epitopes on live cells; this conjugate is suitable for surface staining without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your target B-cell subset; memory B cells, which preferentially express CD307b, may yield brighter staining.
• The conjugate should be compatible with standard 594 nm lasers; always include single-stain controls and a viability dye for accurate gating.
• Validate the conjugate in the relevant biological sample (e.g., human PBMCs or lymphoid tissue) to confirm specificity and performance.
• Reported cross-reactivity is for human; if using other species, experimental verification is recommended.
• Because CD307b expression may vary among B-cell subtypes, include an appropriate isotype control to distinguish specific from non-specific signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials and technical services. For B cell immunology and lymphoma research, we supply validated monoclonal antibodies such as this CD307b conjugate, along with antibody pairs, optimized conjugates, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio provides a streamlined procurement bridge to accelerate your assay development.