Rabbit anti-Human CD307a/FCRL1 Monoclonal Antibody for Flow Cytometry - Q96LA6

Core Product Specifications and Parameters

Biological Background: FCRL1 Function and Localization

• Encodes a type I transmembrane glycoprotein designated Fc receptor-like protein 1 (FCRL1), also known as FcRH1, hIFGP1, IRTA5, and CD307a.
• Regulates B-cell receptor (BCR) signaling, modulating B-cell activation (PubMed:15479727). Related references: PMID:15479727
• Enhances B-cell activation by recruiting the kinase ABL1, while suppressing ERK activation via GRB2 under homeostatic conditions.
• Belongs to the immunoglobulin receptor superfamily, featuring extracellular immunoglobulin-like domains.
• Localizes to the cell membrane as a single-pass transmembrane protein.
• Primarily expressed by mature B cells in secondary lymphoid tissues (e.g., spleen, lymph node), with higher levels in naïve vs. memory B cells; also detected in heart, kidney, liver, and placenta.
• Subject to alternative splicing, producing isoforms observed at 47kDa, 40kDa, and 22kDa (predicted molecular weight of 47kDa).
• Contains intramolecular disulfide bonds and N-linked glycosylation sites essential for receptor structure.

Experimental Guidance and Technical Tips

• Titrate the conjugate to establish an optimal working dilution for your specific cell type and flow cytometry panel.
• Include appropriate isotype control antibodies to assess and adjust for non-specific binding in single- or multicolor experiments.
• Validate specificity using FCRL1‑negative cell lines or knockout controls, especially given the presence of multiple isoforms.
• For multicolor flow cytometry, account for spectral properties and perform proper compensation.
• The immunogen (extracellular domain aa 17‑303) may recognize all major isoforms; verify expected staining pattern in the relevant biological context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, linking diagnostic developers with high-quality antibody reagents and technical support from concept to clinic. This CD307a/FCRL1 antibody supports B-cell immunophenotyping and signaling research, and our supply chain covers validated antibody pairs, bulk ancillary reagents, and custom monoclonal/polyclonal development for rare or challenging targets. Reach out to streamline your procurement workflow and accelerate assay development.