Rabbit anti-Human CD307a/FCRL1 Monoclonal Antibody for FC - Q96LA6

Core Product Specifications and Parameters

Biological Background: Fc receptor-like protein 1 (FCRL1/CD307a) Function and Localization

• FCRL1 (Fc receptor-like protein 1), also known as FcRH1, IFGP1, and IRTA5, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It is preferentially expressed on mature B-cells and functions as a regulator of B-cell antigen receptor (BCR) signaling, fine-tuning B-cell activation and homeostasis. Related references: PMID:15479727
• FCRL1 recruits ABL1 as an intracellular effector to enhance B-cell activation, while simultaneously suppressing ERK activation in a GRB2-dependent manner under both homeostatic and BCR-stimulated conditions (By similarity).
• The protein is localized to the cell membrane, with its extracellular region containing immunoglobulin-like domains likely involved in ligand binding.
• FCRL1 undergoes alternative splicing, producing isoforms with different molecular weights (predicted ~47, 40, and 22 kDa), and is subject to post-translational modifications including glycosylation and phosphorylation.
• Tissue specificity is primarily in secondary lymphoid organs (spleen, lymph node), with higher expression in naive compared to memory B-cells; also detected at low levels in heart, skeletal muscle, kidney, liver, and placenta.

Experimental Guidance and Technical Tips

• The immunogen (aa 17-303) corresponds to the extracellular domain of human FCRL1, ensuring that the antibody binds to the native surface-expressed protein. For flow cytometry applications, this design supports detection of membrane-bound FCRL1 on live cells.
• Consider titrating the antibody to determine the optimal staining concentration for your specific experimental conditions, as staining intensity may vary with cell type and activation state.
• Use appropriate Fc receptor blocking reagents and isotype controls to minimize non-specific binding, especially when analyzing B-cell populations.
• Validate antibody performance in your sample system by including positive (e.g., human B-cell lines or peripheral blood B-cells) and negative (T-cells) controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material provider, connecting diagnostic manufacturers and research laboratories with high-quality antibodies like this FCRL1 clone. We support B-cell immunophenotyping projects by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored rare target sourcing. Let CamelBio streamline your procurement from concept to clinic.