Rabbit anti-Human CD307a/FCRL1 Monoclonal Antibody for FC - Q96LA6

Core Product Specifications and Parameters

Biological Background: FCRL1 Function and Localization

• FCRL1 is a type I transmembrane glycoprotein also known as Fc receptor homolog 1 (FcRH1), IFGP family protein 1, and immune receptor translocation-associated protein 5 (IRTA5).
• Core function: Regulates B-cell receptor (BCR)-mediated signaling responses, enhancing B-cell activation via ABL1 recruitment and suppressing ERK activation in a GRB2-dependent manner. Related references: PMID:15479727
• Member of the Fc receptor-like family, sharing structural features with classical Fc receptors, and plays a role in modulating immune responses.
• Subcellular localization: Cell membrane, consistent with its role as a surface receptor on B-cells.
• Tissue specificity: Primarily expressed in secondary lymphoid tissues by mature B-cell subsets, with higher expression in naive compared to memory B-cells; also detected in spleen, lymph node, heart, skeletal muscle, kidney, liver, and placenta.
• Keywords indicate post-translational modifications: Disulfide bond, glycoprotein, phosphoprotein; and alternative splicing, suggesting multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 17-303) of human FCRL1, which may allow detection of surface-expressed full-length protein as well as potential isoforms.
• conjugation enables direct detection by flow cytometry; consider titrating the antibody for optimal signal-to-noise ratio in your cell system.
• Given expression in B-cells, validate reactivity using human B-cell lines or primary B-cells; naive B-cells may show higher staining.
• Perform appropriate controls (e.g., isotype control, Fc block) to ensure specificity.

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