Rabbit anti-Human CD305/LAIR1 Monoclonal Antibody for Flow Cytometry - Q6GTX8

Core Product Specifications and Parameters

Biological Background: CD305/LAIR1 Function and Localization

• CD305/LAIR1 is a leukocyte-associated immunoglobulin-like receptor that functions as an inhibitory receptor on natural killer (NK) cells, B-cells, and T-cells, playing a constitutive negative regulatory role in cytolytic function.
• Upon activation by tyrosine phosphorylation, it recruits and activates the phosphatases PTPN6 and PTPN11, and reduces intracellular calcium increase evoked by B-cell receptor ligation.
• It modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG while inducing transforming growth factor beta; in B-cells, it down-regulates IgG, IgE, IL8, IL10, and TNF secretion.
• Subcellular localization is primarily at the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue specificity: expressed on the majority of peripheral mononuclear cells, including NK cells, T-cells, B-cells, monocytes, and dendritic cells; highly expressed in naive T- and B-cells but absent from germinal center B-cells.
• Protein features include immunoglobulin domains, disulfide bonds, glycosylation, and phosphorylation sites; the mature protein has a molecular weight of approximately 31.5 kDa, though observed weights vary (23/29/31 kDa) due to alternative splicing and glycosylation.
• Keywords: 3D-structure, Adaptive immunity, Alternative splicing, Cell membrane, Disulfide bond, Glycoprotein, Immunity, Immunoglobulin domain, Membrane, Phosphoprotein, Proteomics identification, Receptor, Reference proteome, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• This antibody is a direct conjugate with , suitable for flow cytometry (FC) analysis of live cells; consider titrating the antibody to determine the optimal staining concentration for your specific sample.
• The immunogen corresponds to the extracellular domain (amino acids 22–163), so it should recognize the native surface receptor; validate staining specificity with appropriate isotype controls and, if possible, blocking peptides or knockout cell lines.
• CD305/LAIR1 is expressed on various immune cell subsets; include unstimulated controls and consider co-staining with lineage markers to identify cell populations of interest.
• Since the protein exhibits multiple molecular weights due to glycosylation and isoforms, use appropriate protein standards if performing complementary biochemical assays; this product is validated for FC, and performance in other applications should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For studies involving immune checkpoint receptors like CD305/LAIR1, we offer comprehensive sourcing solutions—from monoclonal antibodies and antigens to bulk raw materials—supporting assay development from concept to clinical application. Contact us to discuss your specific procurement needs.