Rabbit anti-Human CD304/Neuropilin-1 Monoclonal Antibody for Flow Cytometry - O14786

Core Product Specifications and Parameters

Biological Background: Neuropilin-1 Function and Localization

• Neuropilin-1 (NRP1) is a cell-surface receptor for semaphorins (e.g., Sema3A) and VEGF family members (VEGF165, PLGF-2, VEGFB), mediating axonal guidance, angiogenesis, and cardiovascular development. Related references: PMID:10688880 PMID:9288753 PMID:9529250
• It recognizes a C-end rule (CendR) motif R/KXXR/K on ligands, triggering cellular internalization and vascular leakage. Related references: PMID:19805273
• Coexpression with KDR/VEGFR2 enhances VEGF165 binding and chemotaxis, amplifying VEGF-induced angiogenic signaling.
• NRP1 regulates mitochondrial iron transport through interaction with ABCB8/MITOSUR. Related references: PMID:30623799
• Soluble isoforms (lacking the transmembrane domain) bind VEGF165 and inhibit its cell‑surface binding, potentially inducing apoptosis. Related references: PMID:10748121 PMID:26503042
• Acts as a host factor for SARS‑CoV‑2 infection by binding the CendR motif RRAR on the spike protein, enhancing viral entry.
• Localizes to the cell membrane, cytoplasm, mitochondrion membrane, and is also secreted.
• Highly expressed in heart and placenta; moderate in lung, liver, skeletal muscle, kidney, pancreas; low in adult brain. In development, predominantly in nervous system; also present in olfactory epithelium. Related references: PMID:10688880 PMID:9529250 PMID:33082293

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full extracellular domain (aa 22–644), suggesting the antibody likely recognizes surface‑expressed NRP1; perform live‑cell staining for application in flow cytometry.
• Multiple isoforms and post‑translational modifications result in observed molecular weights ranging from 68 to 103 kDa; validate band patterns in your specific sample system.
• Soluble NRP1 isoforms may interfere if only membrane‑bound detection is desired; consider using appropriate controls or alternative methodologies.
• For functional assays, determine whether the antibody exhibits blocking or neutralizing activity by testing in appropriate ligand‑binding or cellular assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers validated monoclonal antibodies, including this anti‑NRP1 clone, plug‑and‑play antibody pairs, and ancillary reagents for IVD development. Our sourcing network covers rare‑target raw materials, bulk custom preparations, and technical support—from early‑stage research to clinical application. Let CamelBio streamline your procurement for neurobiology, angiogenesis, and infectious disease projects.