Rabbit anti-Human CD304/Neuropilin-1 mAb for FC - O14786

Core Product Specifications and Parameters

Biological Background: Neuropilin-1 Function and Localization

• Neuropilin-1 is a cell-surface receptor pivotal for cardiovascular development, angiogenesis, and neuronal circuit formation. It mediates the chemorepulsive activity of semaphorins, guiding axon growth cones. Related references: PMID:10688880, PMID:9288753, PMID:9529250

• The receptor recognizes a C-end rule (CendR) motif on its ligands, which triggers cellular internalization and vascular leakage. Related references: PMID:19805273

• It binds VEGF165 and placental growth factor-2 (PLGF-2), enhancing VEGF165 signaling through co‑receptor KDR. A soluble isoform sequesters VEGF165 and induces apoptosis. Related references: PMID:10748121, PMID:26503042

• Neuropilin-1 acts as a host factor for SARS‑CoV‑2 infection by interacting with the CendR motif on the spike protein.

• The protein is found at the cell membrane, in the cytoplasm, on the mitochondrial membrane, and is also secreted. It is heavily modified by N‑glycosylation, disulfide bonds, and phosphorylation, and contains heparan sulfate proteoglycan chains.

• In adult tissues, NRP1 is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney, and pancreas; and low in brain. Two isoforms show non‑overlapping tissue distributions.

• Beyond angiogenesis and neurogenesis, NRP1 regulates mitochondrial iron transport via interaction with ABCB8. Related references: PMID:30623799

Experimental Guidance and Technical Tips

• The immunogen corresponds to the entire extracellular domain (amino acids 22–644); the resulting monoclonal antibody likely recognizes native, cell‑surface Neuropilin‑1, making it well‑suited for flow cytometry detection of live or fixed cells.
• Titrate the antibody concentration for optimal signal‑to‑noise ratio, considering the expression level of CD304/Neuropilin‑1 in your target cell population (e.g., high in endothelial cells, some tumor cells).
• Use appropriate isotype controls and, if analyzing recombinant protein, include a blocking step with the immunogen to confirm specificity.
• For multicolor panels, ensure is compatible with your instrument’s filter configuration; the fluorophore has excitation/emission at ~690/713 nm, similar to Alexa Fluor® 700.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw‑material partner, providing researchers and diagnostic developers with validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate assay development from concept to clinic. With expertise spanning angiogenesis, neuroscience, and infectious disease targets, CamelBio supports sourcing of rare and specialized raw materials, including Neuropilin‑1‑related products for COVID‑19 host factor research and beyond.