Rabbit anti-Human CD303/BDCA-2 Monoclonal Antibody for Flow Cytometry - Q8WTT0

Core Product Specifications and Parameters

Biological Background: CD303/BDCA-2 Function and Localization

• CD303/BDCA-2 (CLEC4C) is a C-type lectin receptor primarily expressed on the surface of plasmacytoid dendritic cells (PDCs) and immature monocyte-derived dendritic cells. It functions as an endocytic receptor involved in antigen capture and presentation.
• Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(beta1-3/4)GlcNAc(beta1-2)Man, facilitating ligand internalization into antigen-processing compartments. Related references: PMID:21880719, PMID:25995448
• Efficiently targets captured ligands into peptide-loading compartments for presentation to T-cells, linking innate and adaptive immunity. Related references: PMID:11748283
• Mediates potent inhibition of IFN-alpha/beta induction in plasmacytoid dendritic cells, downmodulating innate antiviral responses. Related references: PMID:11748283, PMID:21880719
• Acts as a signaling receptor by activating protein-tyrosine kinases and mobilizing intracellular calcium upon ligand engagement. Related references: PMID:11748283
• Contains a single C-type lectin domain, a transmembrane helix, and a short cytoplasmic tail. Its extracellular domain harbors disulfide bonds and N-glycosylation sites.
• Localizes to the cell membrane as a type II transmembrane protein. Tissue expression is highly restricted to PDCs and is down-regulated during dendritic cell maturation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 45–213) of human CD303, ensuring recognition of the native surface receptor. For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and instrument settings.
• Use appropriate isotype controls and include Fc receptor blocking steps to minimize nonspecific binding when staining human peripheral blood mononuclear cells or purified dendritic cells.
• This conjugate allows direct detection without a secondary antibody. Consider fixation/permeabilization protocols if intracellular staining is required, but validate that the epitope is preserved.
• The antibody is suitable for immunophenotyping plasmacytoid dendritic cells in human samples. For rare cell populations, pre-enrichment strategies may improve signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this CD303/BDCA-2 monoclonal antibody as part of a comprehensive portfolio of immunology reagents for dendritic cell and innate immunity research. As a one-stop sourcing partner, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement to diagnostic manufacturers, labs, and research institutes worldwide. From concept to clinic, our integrated supply chain accelerates your IVD development with reliable, high-quality raw materials.