Rabbit anti-Human CD303/BDCA-2 Monoclonal Antibody for Flow Cytometry - Q8WTT0

Core Product Specifications and Parameters

Biological Background: CD303/BDCA-2 Function and Localization

• CD303/BDCA-2 is a transmembrane C-type lectin receptor (CLEC4C) primarily expressed on plasmacytoid dendritic cells, where it mediates antigen capture and presentation to T cells. Related references: PMID:11748283 PMID:21880719 PMID:25995448
• The receptor specifically binds non-sialylated galactose-terminated biantennary glycans, particularly those containing the Gal(β1-3/4)GlcNAc(β1-2)Man trisaccharide epitope, as well as serum IgG. Related references: PMID:21880719 PMID:25995448
• Upon ligand binding, CD303/BDCA-2 efficiently targets antigens into processing and peptide-loading compartments for MHC-mediated presentation. Related references: PMID:11748283
• Activation of CD303/BDCA-2 potently inhibits the induction of IFN-α/β expression in PDCs, suggesting a regulatory role in innate antiviral responses. Related references: PMID:11748283 PMID:21880719
• The receptor functions as a signaling molecule, capable of activating protein-tyrosine kinases and mobilizing intracellular calcium. Related references: PMID:11748283
• CD303/BDCA-2 is localized to the cell membrane and is constitutively expressed in immature monocyte-derived dendritic cells, with expression significantly down-regulated upon LPS-induced maturation.
• The protein is characterized by the presence of a C-type lectin domain, a transmembrane helix, and a signal-anchor sequence; it undergoes alternative splicing and N-linked glycosylation.
• Predicted molecular weight of the unmodified protein is approximately 25 kDa, with glycosylated forms migrating at around 21-25 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 45-213 of human CD303/BDCA-2, which includes the extracellular C-type lectin domain; the resulting antibody is expected to recognize native and denatured forms of the target.
• This -conjugated monoclonal antibody is validated for flow cytometry (FC) applications; titrate the antibody to determine the optimal working concentration for your specific experimental conditions.
• For immunophenotyping of plasmacytoid dendritic cells, consider co-staining with other PDC markers and appropriate isotype controls.
• Ensure proper sample preparation and viability to minimize non-specific binding; use freshly isolated or properly cryopreserved human PBMCs or purified dendritic cells.
• Avoid prolonged exposure to light and store the antibody at 2-8°C protected from light.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, bulk reagents, and customized procurement services to support diagnostic development and biomedical research. This anti-CD303/BDCA-2 antibody serves as a critical tool for dendritic cell immunophenotyping and immune monitoring applications. Explore our comprehensive portfolio of antibody pairs, ancillary reagents, and rare target resources to streamline your diagnostic workflow from concept to clinic.