Rabbit anti-Human CD303/BDCA-2 Monoclonal Antibody for Flow Cytometry - Q8WTT0

Core Product Specifications and Parameters

Biological Background: CD303/BDCA-2 Function and Localization

• CD303/BDCA-2 is a C-type lectin domain family 4 member C, also known as blood dendritic cell antigen 2, primarily expressed on plasmacytoid dendritic cells (PDCs).
• It functions as a lectin-type cell surface receptor that mediates antigen capture and presentation to T cells, playing a key role in adaptive immunity. Related references: PMID:11748283, PMID:21880719, PMID:25995448
• Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(β1-3/4)GlcNAc(β1-2)Man. Related references: PMID:21880719, PMID:25995448
• Binds to serum IgG, which facilitates efficient targeting of ligands into antigen-processing and peptide-loading compartments. Related references: PMID:25995448
• Potently inhibits induction of IFN-α/β expression in PDCs, modulating innate antiviral responses. Related references: PMID:11748283, PMID:21880719
• Acts as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium upon ligand engagement. Related references: PMID:11748283
• Located on the cell membrane, consistent with its role in extracellular ligand sensing and endocytic trafficking.
• Constitutively expressed in immature monocyte-derived dendritic cells; significantly down-regulated upon LPS-induced maturation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 45–213 of the extracellular domain, so the antibody is expected to recognize surface CD303/BDCA-2 in live-cell flow cytometry.
• Conjugated to , a bright fluorophore suitable for multi-color panels; verify compatibility with your cytometer’s laser and filter configuration.
• As a monoclonal antibody, it provides high specificity; however, users should titrate the optimal working dilution and include proper isotype controls in their experiments.
• For intracellular staining, fixation and permeabilization conditions should be tested, as epitope accessibility may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop provider of IVD raw materials, supporting diagnostic developers and researchers with a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This anti-CD303/BDCA-2 monoclonal antibody is part of our immune cell marker collection, enabling accurate detection of plasmacytoid dendritic cells in flow cytometry workflows. Beyond catalog products, we offer custom antibody development, bulk supply, and technical consulting to accelerate your projects from concept to clinic.