Rabbit anti-Human CD303/BDCA-2 Monoclonal Antibody for FC - Q8WTT0

Core Product Specifications and Parameters

Biological Background: CLEC4C/CD303/BDCA-2 Function and Localization

• CD303/BDCA-2 (C-type lectin domain family 4 member C, CLEC4C) is a type II transmembrane glycoprotein belonging to the C-type lectin superfamily, and serves as a specific marker for human plasmacytoid dendritic cells (PDCs).
• The receptor specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(β1-3/4)GlcNAc(β1-2)Man, and binds to serum IgG. Related references: PMID:21880719, PMID:25995448
• BDCA-2 efficiently targets captured ligands into antigen-processing and peptide-loading compartments, facilitating presentation to T cells, thus playing a role in adaptive immunity. Related references: PMID:11748283
• Ligation of BDCA-2 potently inhibits the induction of type I interferon (IFN-α/β) expression in plasmacytoid dendritic cells, linking this receptor to innate antiviral responses. Related references: PMID:11748283, PMID:21880719
• BDCA-2 can act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium, suggesting involvement in downstream signaling cascades. Related references: PMID:11748283
• The protein is localized at the cell membrane via a signal-anchor transmembrane domain and contains extracellular C-type lectin domains stabilized by disulfide bonds; it binds calcium, which is required for carbohydrate recognition.
• Expression is restricted to plasmacytoid dendritic cells and immature monocyte-derived dendritic cells, with significant downregulation upon maturation induced by LPS but not by TNF.
• A naturally occurring alternative splicing isoform may exist, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The recombinant immunogen spans the extracellular domain (amino acids 45–213), which includes the carbohydrate recognition domain; consider that reduced or denatured samples may affect antibody binding due to disulfide-dependent epitopes.
• This conjugate is designed for flow cytometry; validate the antibody on relevant plasmacytoid dendritic cell populations using appropriate surface markers (e.g., CD123, CD4, CD45RA).
• Because BDCA-2 expression is downregulated upon LPS maturation, avoid using LPS-stimulated cells as a positive control; freshly isolated or steady-state PDCs are recommended.
• excites at ~650 nm and emits in the far-red region; verify instrument laser and filter configurations, and include appropriate isotype controls for gating.
• For intracellular applications or detection of the alternative isoform, additional validation in the specific experimental system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials. For CD303/BDCA-2 (CLEC4C) research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing services also support rare target raw-material procurement, ensuring you have reliable access to critical components for dendritic cell and immunology applications.