Rabbit anti-Human CD301/CLEC10A Monoclonal Antibody for FC - Q8IUN9

Core Product Specifications and Parameters

Biological Background: CLEC10A Function and Localization

• CLEC10A is a type II transmembrane C-type lectin receptor with alternative splicing isoforms, also known as CD301, macrophage galactose-type lectin (MGL), HML, and DC-asialoglycoprotein receptor, expressed predominantly on myeloid antigen presenting cells.
• It specifically binds terminal N-acetylgalactosamine (GalNAc) residues present on glycoproteins and glycolipids, including Tn antigen, LacdiNAc, and GM2/GD2 gangliosides, in a Ca2+-dependent manner. Related references: PMID:15802303, PMID:23507963
• On immature dendritic cells, CLEC10A interacts with Tn antigen-modified CD45 on T cells, downregulating its phosphatase activity to modulate T cell activation, proliferation, and cytokine production, promoting tolerogenic dendritic cell maturation and regulatory T cell induction. Related references: PMID:15802303, PMID:16998493, PMID:22213806
• As an endocytic receptor, CLEC10A internalizes Tn antigen-bearing tumor antigens for MHC class I and II presentation, linking innate recognition to adaptive antitumor immune responses. Related references: PMID:15802303, PMID:17616966, PMID:17804752
• Subcellularly, CLEC10A localizes to the plasma membrane and, upon ligand binding, traffics to early endosome and lysosome membranes for antigen processing.
• At the protein level, CLEC10A is expressed in myeloid antigen presenting cells in lymph nodes and skin, specifically in dermal dendritic cells.
• The protein contains a coiled-coil domain, a C-type lectin domain, a transmembrane helix, and potential N-glycosylation sites; its activity is regulated by Ca2+ and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 61-316) including the carbohydrate recognition domain (CRD), indicating the antibody likely targets the ligand-binding region; consider verifying structural epitope integrity in native versus denaturing conditions.
• For flow cytometry, this fluorescent conjugate can be used to detect cell surface CLEC10A on human myeloid cells; titrate the antibody and include appropriate isotype controls to ensure specificity.
• Cross-reactivity with human CLEC10A has been reported; validate reactivity in your specific sample system, especially considering potential isoform variations.
• Use ice-cold buffer and avoid permeabilization unless targeting intracellular compartments, as the receptor is membrane-bound; include Fc receptor blocking steps for myeloid cell analysis.
• Consider co-staining with dendritic cell markers (e.g., CD11c, CD1c) to confirm expression on dendritic cell subsets; note that activation status may alter CLEC10A expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier provider of IVD raw materials, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. This anti-CLEC10A monoclonal antibody supports research into immune recognition and dendritic cell biology, with CamelBio ensuring reliable supply and technical support for your immunoassay development. For rare-target sourcing or customized conjugation needs, our one-stop platform simplifies your transition from concept to clinic.