ABflo 488 Rabbit anti-Human CD301/CLEC10A Monoclonal Antibody for FC - Q8IUN9

Core Product Specifications and Parameters

Biological Background: CD301/CLEC10A Function and Localization

• CD301/CLEC10A functions as a C-type lectin receptor that specifically recognizes N-acetylgalactosamine (GalNAc)-terminated glycans on glycoproteins and glycolipids in a Ca²⁺-dependent manner, including Tn antigen, T antigen, and LacdiNAc epitopes. Related references:
  PMID:15802303 | PMID:16998493 | PMID:17616966 | PMID:23507963

• It acts as a signaling receptor at the interface of APC–T cell interactions, downregulating CD45 phosphatase activity on effector T cells and promoting dendritic cell tolerance, which leads to the generation of immunosuppressive regulatory CD4⁺ T cells. Related references:
  PMID:15802303 | PMID:16998493 | PMID:22213806

• As an endocytic pattern recognition receptor, CD301/CLEC10A internalizes Tn antigen–carrying tumor mucins and delivers them to endolysosomal compartments for MHC class I and II antigen presentation, contributing to antitumor immunity. Related references:
  PMID:15802303 | PMID:17616966 | PMID:17804752

• CD301/CLEC10A localizes to the cell membrane, early endosome membrane, and lysosome membrane, consistent with its roles in ligand binding, internalization, and antigen processing.

• The protein is predominantly expressed in myeloid antigen-presenting cells, including dermal dendritic cells in lymph nodes and skin.

• Alternative splicing generates multiple isoforms of CD301/CLEC10A, contributing to functional diversity; the canonical isoform has a predicted molecular mass of ~35 kDa, with additional bands observed at 28 kDa and 32 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 61–316) encompasses a large portion of the extracellular domain, including the carbohydrate recognition domain (CRD). This design likely generates antibodies that recognize native CD301/CLEC10A in flow cytometry.

• ABflo 488 conjugation provides a direct fluorescent label for single-step staining; always include appropriate isotype controls and fluorescence-minus-one (FMO) controls to confirm specificity.

• For FC, optimize antibody titrations using a relevant cell line or primary myeloid dendritic cells; a typical starting concentration range is 0.5–5 µg per million cells, but this should be empirically determined.

• Since CD301/CLEC10A is expressed on myeloid DCs and macrophages, consider using PBMCs or monocyte-derived dendritic cells for validation; verify expression by co-staining with lineage markers (e.g., CD14, CD1c).

• The antibody may also be suitable for detecting tumor-infiltrating dendritic cells in dissociated tumor samples; validate fixation and permeabilization protocols if intracellular staining is required.

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