Rabbit anti-Human CD301/CLEC10A mAb for FC - Q8IUN9

Core Product Specifications and Parameters

Biological Background: CD301/CLEC10A Function and Localization

• CLEC10A (also designated HML, MGL, HML2, CD301, CLECSF13, CLECSF14) is a type II transmembrane C-type lectin receptor encoded by the CLEC10A gene in humans.
• CLEC10A binds GalNAc-terminated glycans in a Ca²⁺-dependent manner, including Tn antigen (GalNAc-O-Ser/Thr), T antigen (Galβ1-3GalNAc), LacdiNAc (GalNAcβ1-4GlcNAc), core 5/6 O-glycans, and gangliosides GM2/GD2, enabling recognition of tumor-associated carbohydrate antigens. Related references: PMID:15802303 PMID:23507963
• Acts as a signaling receptor at the interface of APC–T cell interactions: it recognizes Tn antigen on PTPRC/CD45 of effector T cells, downregulates CD45 phosphatase activity, and modulates T cell activation threshold, cytokine production, and proliferation. Related references: PMID:15802303 PMID:16998493 PMID:22213806
• Functions as an endocytic pattern recognition receptor that internalizes Tn‑terminated immunogens and targets them to endolysosomal compartments for MHC class I and II antigen presentation, contributing to antitumor immunity. Related references: PMID:15802303 PMID:17616966 PMID:17804752
• Localizes to the cell membrane, early endosome membrane, and lysosome membrane, consistent with its roles in ligand binding, signaling, and internalization.
• Expressed primarily in myeloid antigen‑presenting cells, including dermal dendritic cells and lymph node APCs, as detected at the protein level.
• Contains a coiled‑coil domain and disulfide bonds, and is N‑glycosylated. The canonical UniProt sequence predicts a molecular weight of ~35 kDa, while observed variants run at 28, 32, and 35 kDa, likely reflecting alternative splicing or differential glycosylation.
• Multiple isoforms are produced by alternative splicing, which may account for the observed molecular weight heterogeneity.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a far‑red fluorescent dye, allowing single‑step staining for flow cytometry without a secondary reagent. Titrate the antibody to determine the optimal staining concentration for your experimental system.
• The immunogen spans the extracellular domain (amino acids 61–316); therefore, the antibody is suitable for live‑cell surface staining without permeabilization. Confirm binding under native conditions in the relevant cell type.
• When designing multicolor flow cytometry panels, verify spectral compatibility of with other fluorophores used. Preblocking of Fc receptors on myeloid cells with appropriate blocking reagents may reduce nonspecific binding.
• Include appropriate isotype controls and fluorescence minus one (FMO) controls to assess background staining and define positive populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for IVD raw materials, connecting diagnostic developers with well‑characterized reagents like this fluorescently labeled CD301/CLEC10A monoclonal antibody. For your immunology and oncology assay development, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare‑target raw‑material procurement. We help you move from concept to clinic with reliable, research‑grade inputs.